Transportation of whey protein-derived peptides using Caco-2 cell model and identification of novel cholesterol-lower ing peptides
文献类型： 外文期刊
作者： Liu, Mingzhen 1 ; Liu, Feifan 1 ; Zhang, Tao 1 ; Zhao, Xuan 1 ; Wang, Xiaozhi 1 ; Kong, Weimei 1 ; Cui, Li 2 ; Luo, Haibo 1 ; Guo, Lili 3 ; Guo, Yuxing 1 ;
作者机构： 1.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Dept Food Sci & Technol, Nanjing, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing, Peoples R China
3.Shanxi Univ Chinese Med, Coll Tradit Chinese Med & Food Engn, Jinzhong, Peoples R China
4.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210097, Peoples R China
5.Shanxi Univ Chinese Med, Coll Traditional Chinese Med & Food Engn, Jinzhong 030600, Peoples R China
关键词： whey protein; enzymatic hydrolysis; cholesterol-lowering peptides; Caco-2 cells; intestinal transport; gastrointestinal digestion
期刊名称：FOOD & NUTRITION RESEARCH （ 影响因子：3.3； 五年影响因子：5.0 ）
ISSN： 1654-6628
年卷期： 2023 年 67 卷
页码：
收录情况： SCI
摘要： Background: The increasing morbidity and mortality of cardiovascular disease have become a major factor in human death. Serum cholesterol is considered to be an important risk factor for inducing coronary heart disease, atherosclerosis and other cardiovascular diseases. To screen intestinal absorbable functional small peptides with cholesterol-lowering activity by enzymatic hydrolysis of whey protein and develop cholester-ol-based functional food that may become a substitute for chemically synthesized drugs, providing new ideas for diseases caused by high cholesterol.Objective: This study aimed to evaluate the cholesterol-lowering activity of intestinal absorbable whey pro-tein-derived peptides hydrolyzed by alkaline protease, trypsin and chymotrypsin, respectively.Method: The whey protein hydrolysates acquired by enzymatic hydrolysis under optimal conditions were puri-fied by a hollow fiber ultrafiltration membrane with a molecular weight cutoff of 10 kDa. The fractions obtained by Sephadex G-10 gel filtration chromatography were transported through a Caco-2 cell mono-layer. The transported peptides were detected in the basolateral aspect of Caco-2 cell monolayers using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS).Results: His-Thr-Ser-Gly-Tyr (HTSGY), Ala-Val-Phe-Lys (AVFK) and Ala-Leu-Pro-Met (ALPM) were unre-ported peptides with cholesterol-lowering activity. The cholesterol-lowering activities of the three peptides did not change significantly during simulated gastrointestinal digestion.Conclusion: This study not only provides theoretical support for the development of bioactive peptides that can be directly absorbed by the human body, but also provides new treatment ideas for hypercholesterolemia.
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