Immune Enhancement of Nanoparticle-Encapsulated Ginseng Stem-Leaf Saponins on Porcine Epidemic Diarrhea Virus Vaccine in Mice
文献类型： 外文期刊
作者： Su, Fei 1 ; Xu, Lihua 1 ; Xue, Yin 2 ; Xu, Wei 3 ; Li, Junxing 1 ; Yu, Bin 1 ; Ye, Shiyi 1 ; Yuan, Xiufang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310002, Peoples R China
2.Zhejiang Ctr Anim Dis Control, Hangzhou 310020, Peoples R China
3.Zhejiang Univ, Coll Anim Sci, Ctr Vet Sci, Zhejiang Prov Key Lab Prevent Vet Med,MOA Key Lab, Hangzhou 310058, Peoples R China
关键词： ginseng stem-leaf saponins; nanoparticles; mucosal adjuvant; porcine epidemic diarrhea virus
期刊名称：VACCINES （ 影响因子：7.8； 五年影响因子：7.4 ）
ISSN：
年卷期： 2022 年 10 卷 11 期
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea virus (PEDV) causes severe enteric disease in pigs, particularly neonatal piglets. Current vaccines do not provide complete protection against PEDV. Ginseng stem-leaf saponins (GSLS), a promising oral adjuvant candidate, can improve intestinal immune responses in poultry and mice. However, its low stability limits further use. Poly lactic-co-glycolic acid (PLGA), a biocompatible and biodegradable nanoparticle, has been widely used in biomedicine for stable and targeted drug delivery. In this study, we developed GSLS-PLGA nanoparticles (GSLS-NPs) and evaluated the mucosal adjuvant efficacy in vitro and in vivo. GSLS-NPs significantly enhanced antigen internalization and pro-inflammatory cytokine secretion by DC2.4 cells. Mice orally administered GSLS-NPs before intramuscular inoculation generated CD11b(+)CD8 alpha(-) and CD11b(-)CD103(+) dendritic cells in the spleen and draining mesenteric lymph nodes, respectively, which are the types mainly responsible for antigen presentation. Additionally, enhanced neutralizing and non-neutralizing antibody responses and expanded activities of specific effector and memory CD4(+) and CD8(+) T cells were also observed in mice immunized with PEDV vaccines plus GSLS-NPs compared to mice receiving the vaccines alone. Furthermore, GSLS-NPs showed a good safety profile and presented great advantages over GSLS aqueous solution. Collectively, our results highlight the potential of GSLS-NPs as a mucosal adjuvant and provide an attractive vaccination strategy for combatting PEDV. Further study is required to evaluate the efficacy of this mucosal adjuvant in swine.
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