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Schizolysin, a hemolysin from the split gill mushroom Schizophyllum commune

文献类型： 外文期刊

作者： Han, Chun-Hua 2 ; Zhang, Guo-Qing 3 ; Wang, He-Xiang 3 ; Ng, Tzi Bun 1 ;

作者机构： 1.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Anim & Husb Med, Beijing, Peoples R China

3.China Agr Univ, Dept Microbiol, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

关键词： hemolysin;mushroom;purification;Schizophyllum commune

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN：

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收录情况： SCI

摘要： A monomeric hemolysin with a molecular mass of 29 kDa was isolated from fresh fruiting bodies of the split gill mushroom Schizophyllum commune. The hemolysin was purified by successive adsorption on DEAE-cellulose, carboxymethyl-cellulose and Q-Sepharose and finally gel filtration on Superdex 75. This demonstrated the N-terminal sequence ATNYNKCPGA, different from those of previously reported fungal and bacterial hemolysins. The hemolysin was stable up to 40 °C. Only partial activity remained at 50 and 60 °C. Activity was indiscernible at 70 °C. A pH of 6.0 was optimal for activity. The hemolytic activity was most potently inhibited by dithiothreitol, sucrose and raffinose, followed by cellobiose, maltose, rhamnose, inulin, lactose, fructose and inositol. The metal ions Cu~(2+), Mg~(2+), Zn~(2+), Al~(3+) and Fe~(3+) significantly, and Pb~(2+) to a lesser extent, curtailed the activity of the hemolysin. The hemolysin inhibited HIV-1 reverse transcriptase with an IC_(50) of 1.8 μM.

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