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Expression of Smyd1b_tv1 by Alternative Splicing in Cardiac Muscle is Critical for Sarcomere Organization in Cardiomyocytes and Heart Function

文献类型： 外文期刊

作者： Xu, Rui 1 ; Li, Siping 1 ; Chien, Chien-Ju 1 ; Zhong, Yongwang 3 ; Xiao, Huanhuan 1 ; Fang, Shengyun 3 ; Du, Shaojun 1 ;

作者机构： 1.Univ Maryland, Sch Med, Inst Marine & Environm Technol, Dept Biochem & Mol Biol, Baltimore, MD 21250 USA

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai, Peoples R China

3.Univ Maryland, Sch Med, Ctr Biomed Engn, Dept Physiol, Baltimore, MD USA

关键词： Smyd1; sarcomere; cardiomyocyte; alternative splicing

期刊名称：MOLECULAR AND CELLULAR BIOLOGY （ 影响因子：2.7； 五年影响因子：4.3 ）

ISSN： 0270-7306

年卷期： 2024 年 44 卷 12 期

页码：

收录情况： SCI

摘要： Smyd1, a member of the Smyd lysine methyltransferase family, plays an important role in myofibrillogenesis of skeletal and cardiac muscles. Loss of Smyd1b (a Smyd1 ortholog) function in zebrafish results in embryonic death from heart malfunction. smyd1b encodes two isoforms, Smyd1b_tv1 and Smyd1b_tv2, differing by 13 amino acids due to alternative splicing. While smyd1 alternative splicing is evolutionarily conserved, the isoform-specific expression and function of Smyd1b_tv1 and Smyd1b_tv2 remained unknown. Here we analyzed their expression and function in skeletal and cardiac muscles. Our analysis revealed expression of smyd1b_tv1 predominately in cardiac and smyd1b_tv2 in skeletal muscles. Using zebrafish models expressing only one isoform, we demonstrated that Smyd1b_tv1 is essential for cardiomyocyte differentiation and fish viability, whereas Smyd1b_tv2 is dispensable for heart development and fish survival. Cellular and biochemical analyses revealed that Smyd1b_tv1 differs from Smyd1b_tv2 in protein localization and binding with myosin chaperones. While Smyd1b_tv2 diffused in the cytosol of muscle cells, Smyd1b_tv1 was localized to M-lines and essential for sarcomere organization in cardiomyocytes. Co-IP analysis revealed a stronger binding of Smyd1b_tv1 with chaperones and cochaperones compared with Smyd1b_tv2. Collectively, these findings highlight the nonequivalence of Smyd1b isoforms in cardiomyocyte differentiation, emphasizing the critical role of Smyd1b_tv1 in cardiac function.

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