Cytochrome P450 mono-oxygenase CYP703A2 plays a central role in sporopollenin formation and ms5ms6 fertility in cotton
文献类型： 外文期刊
作者： Ma, Huanhuan 1 ; Wu, Yuanlong 1 ; Lv, Ruiling 2 ; Chi, Huabin 1 ; Zhao, Yunlong 1 ; Li, Yanlong 1 ; Liu, Hongbo 1 ; Ma, Yizan 1 ; Zhu, Longfu 1 ; Guo, Xiaoping 3 ; Kong, Jie 4 ; Wu, Jianyong 5 ; Xing, Chaozhu 5 ; Zhang, Xianlong 1 ; Min, Ling 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
2.Huanggang Normal Univ, Coll Biol & Agr Resources, Huanggang 438000, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Econ Crops, Urumqi 830091, Xinjiang, Peoples R China
5.Chinese Acad Agr Sci, State Key Lab Cotton Biol, Inst Cotton Res, Anyang 455000, Peoples R China
关键词： cotton; cytochrome P450 mono-oxygenase; double-recessive genic male sterility; sporopollenin formation
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）
ISSN： 1672-9072
年卷期： 2022 年 64 卷 10 期
页码：
收录情况： SCI
摘要： The double-recessive genic male-sterile (ms) line ms5 ms6 has been used to develop cotton (Gossypium hirsutum) hybrids for many years, but its molecular-genetic basis has remained unclear. Here, we identified the Ms5 and Ms6 loci through map-based cloning and confirmed their function in male sterility through CRISPR/Cas9 gene editing. Ms5 and Ms6 are highly expressed in stages 7-9 anthers and encode the cytochrome P450 mono-oxygenases CYP703A2-A and CYP703A2-D. The ms5 mutant carries a single-nucleotide C-to-T nonsense mutation leading to premature chain termination at amino acid 312 (GhCYP703A2-A(312aa)), and ms6 carries three nonsynonymous substitutions (D98E, E168K, and G198R) and a synonymous mutation (L11L). Enzyme assays showed that GhCYP703A2 proteins hydroxylate fatty acids, and the ms5 (GhCYP703A2-A(312aa)) and ms6 (GhCYP703A2-D-D98E,D-E168K,D-G198R) mutant proteins have decreased enzyme activities. Biochemical and lipidomic analyses showed that in ms5 ms6 plants, C12-C18 free fatty acid and phospholipid levels are significantly elevated in stages 7-9 anthers, while stages 8-10 anthers lack sporopollenin fluorescence around the pollen, causing microspore degradation and male sterility. Overall, our characterization uncovered functions of GhCYP703A2 in sporopollenin formation and fertility, providing guidance for creating male-sterile lines to facilitate hybrid cotton production and therefore exploit heterosis for improvement of cotton.
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