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Identification and structural analysis of dimeric chicken complement component 3d and its binding with chicken complement receptor 2

文献类型： 外文期刊

作者： Jin, Huan 1 ; Tu, Min 1 ; Meng, Zhaoying 2 ; Jiang, Bo 1 ; Yang, Qianqian 2 ; Li, Yongqing 1 ; Zhang, Zhenhua 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing, Peoples R China

2.Beijing Univ Agr, Anim Sci & Technol Coll, Beijing, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing, Peoples R China

关键词： Chicken complement component 3d; Dimer; Homologous modeling; Chicken complement receptor 2; Surface plasmon resonance; Molecular docking

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0145-305X

年卷期： 2024 年 152 卷

页码：

收录情况： SCI

摘要： Complement component 3d (C3d), the final cleavage product of complement component C3, interacts with CR2 and thus plays a crucial role in linking the innate and adaptive immune systems. Additionally, human C3d executes various functions in its dimeric form, which is more effective than its monomeric form. In this study, we aimed to explored whether chicken C3d (chC3d) exhibits similar characteristics, namely dimerization and binding of dimeric chC3d to chicken CR2 (chCR2). We investigated the interaction and co-localization of chC3d with itself using coimmunoprecipitation and confocal laser scanning microscopy, respectively. Then, dimeric chC3d was detected using native polyacrylamide gel electrophoresis and western blotting, and its equilibrium dissociation constant KD (827 nM) was determined using surface plasmon resonance. Finally, the interaction modes of dimeric chC3d were identified using molecular docking simulations, which revealed that dimeric chC3d could crosslink with chCR2 receptor. Overall, our findings will facilitate future explorations of the chicken complement system.

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