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Development and evaluation of a time-resolved fluorescence labelled immunochromatographic strip assay for rapid and quantitative detection of bovine herpesvirus 1

文献类型： 外文期刊

作者： Liu, Wenxiao 1 ; Zhang, Kun 1 ; Cheng, Jing 1 ; Yu, Shiqiang 4 ; Cheng, Chunjie 1 ; Jiang, Bo 1 ; Zhou, Linyi 1 ; Li, Yongqing 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Res Ctr Infect Dis Livestock & Poultry, Beijing, Peoples R China

3.Beijing Univ Agr, Anim Sci & Technol Coll, Beijing, Peoples R China

4.Nanjing Agr Univ, Coll Anim Sci & Technol, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol,Jiangsu Key Lab Gas, Nanjing, Jiangsu, Peoples R China

关键词： bovine herpesvirus 1; diagnosis; lateral flow strip; time-resolved fluorescent microspheres; monoclonal antibody

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： Bovine herpes virus 1 (BoHV-1) causes a wide variety of diseases in wild and domestic cattle. The most widely used method for viral identification is real-time PCR, which can only be performed in laboratories using sophisticated instruments by expert personnel. Herein, we developed an ultrasensitive time-resolved fluorescence lateral flow immunochromatographic strip (ICS) assay for detecting BoHV-1 in bovine samples using a monoclonal antibody against BoHV-1 labelled with fluorescent microspheres, which can be applied in any setting. The intact process from sample collection to final result can be achieved in 15 min. The limit of detection of the assay for BoHV-1 was 102 TCID50/100 mu L. The coincidence rate of the ICS method and real-time PCR recommended by the World Organization for Animal Health (WOAH) was 100% for negative, 92.30% for positive, and 95.42% for total, as evaluated by the detection of 131 clinical samples. This detection method was specifically targeted to BoHV-1, not exhibiting cross-reactivity with other bovine pathogens including BoHV-5. We developed an ICS assay equipped with a portable instrument that offers a sensitive and specific platform for the rapid and reliable detection of BoHV-1 in the field. The Point-of-Care test of BoHV-1 is suitable for the screening and surveillance of BoHV-1 in dairy herds.

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