Overexpression of an aquaporin protein from Aspergillus glaucus confers salt tolerance in transgenic soybean
文献类型： 外文期刊
作者： Li, Feiwu 1 ; Ni, Hejia 3 ; Yan, Wei 2 ; Xie, Yanbo 2 ; Liu, Xiaodan 4 ; Tan, Xichang 2 ; Zhang, Ling 2 ; Zhang, Shi-Hong 1 ;
作者机构： 1.Jilin Univ, Coll Plant Sci, 5333 Xian Str, Changchun 130062, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, 1363 Shengtai Str, Changchun 130033, Jilin, Peoples R China
3.Northeast Agr Univ, Coll Agr, Harbin 150036, Peoples R China
4.Jilin Agr Sci & Technol Coll, Inst Bioengn, Jilin 132101, Jilin, Peoples R China
关键词： AgGlpF gene; Transgenic soybean; Salt tolerance
期刊名称：TRANSGENIC RESEARCH （ 2020影响因子：2.788； 五年影响因子：2.377 ）
ISSN： 0962-8819
年卷期： 2021 年 30 卷 6 期
收录情况： SCI
摘要： Salt stress is an important abiotic factor that causes severe losses in soybean yield and quality. Therefore, breeding salt-tolerant soybean germplasm resources via genetic engineering has gained importance. Aspergillus glaucus, a halophilic fungus that exhibits significant tolerance to salt, carries the gene AgGlpF. In this study, we used the soybean cotyledonary node transformation method to transfer the AgGlpF gene into the genome of the soybean variety Williams 82 to generate salt-tolerant transgenic soybean varieties. The results of PCR, Southern blot, ddPCR, and RT-PCR indicated that AgGlpF was successfully integrated into the soybean genome and stably expressed. When subjected to salt stress conditions via treatment with 250 mM NaCl for 3 d, the transgenic soybean plants showed significant tolerance compared with wild-type plants, which exhibited withering symptoms and leaf abscission after 9 d. The results of this study indicated that the transfer of AgGlpF into the genome of soybean plants produced transgenic soybean with significantly improved salt stress tolerance.
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