A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis
文献类型： 外文期刊
作者： Dong, Liming 1 ; Li, Congcong 1 ; Xing, Zhenjuan 1 ; He, Yuxuan 1 ; Xia, Wei 1 ; Liu, Yijun 2 ; Long, Likun 1 ; Li, Feiwu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun 130033, Peoples R China
2.Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China
关键词： Genetically modified soybean; High-throughput detection; Single universal primer multiplex PCR; Capillary electrophoresis
期刊名称：JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY （ 影响因子：1.4； 五年影响因子：1.7 ）
ISSN： 1661-5751
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Genetically modified (GM) crops and their products are regulated in many countries. To meet increasing regulatory requirements for a growing number of GM soybean events, high-throughput and cost-effective detection methods are indispensable. In this study, 14 widely commercialized GM soybean events, including GTS40-3-2, MON89788, CV127, A2704-12, A5547-127, 305423, 356043, MON87701, MON87705, MON87708, MON87769, SYHT0H2, FG72, and DAS-444 & Oslash;6-6 were utilized as targets for event-specific identification. A high-throughput detection method was established by integrating a single universal primer multiplex PCR (SUP-M-PCR) with capillary electrophoresis technique. This method enables the identification of 14 soybean events in a single PCR reaction, addressing the limitations of conventional multiplex PCR such as self-inhibition and amplification variations between different primers. Furthermore, it significantly enhances the sensitivity and accuracy of previous multiple detection methods, achieving a detection sensitivity of 0.05% (w/w). Overall, this approach greatly improves detection efficiency and holds promising potential for the identification and detection of 14 types of GM soybean events and their derivatives.
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