A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis
文献类型： 外文期刊
作者： Dong, Liming 1 ; Li, Congcong 1 ; Xing, Zhenjuan 1 ; He, Yuxuan 1 ; Xia, Wei 1 ; Liu, Yijun 2 ; Long, Likun 1 ; Li, Feiwu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun 130033, Peoples R China
2.Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China
关键词： Genetically modified soybean; High-throughput detection; Single universal primer multiplex PCR; Capillary electrophoresis
期刊名称：JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY （ 影响因子：1.4； 五年影响因子：1.7 ）
ISSN： 1661-5751
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Genetically modified (GM) crops and their products are regulated in many countries. To meet increasing regulatory requirements for a growing number of GM soybean events, high-throughput and cost-effective detection methods are indispensable. In this study, 14 widely commercialized GM soybean events, including GTS40-3-2, MON89788, CV127, A2704-12, A5547-127, 305423, 356043, MON87701, MON87705, MON87708, MON87769, SYHT0H2, FG72, and DAS-444 & Oslash;6-6 were utilized as targets for event-specific identification. A high-throughput detection method was established by integrating a single universal primer multiplex PCR (SUP-M-PCR) with capillary electrophoresis technique. This method enables the identification of 14 soybean events in a single PCR reaction, addressing the limitations of conventional multiplex PCR such as self-inhibition and amplification variations between different primers. Furthermore, it significantly enhances the sensitivity and accuracy of previous multiple detection methods, achieving a detection sensitivity of 0.05% (w/w). Overall, this approach greatly improves detection efficiency and holds promising potential for the identification and detection of 14 types of GM soybean events and their derivatives.
- 相关文献
作者其他论文 更多>>
-
A Multiplex PCR System for the Detection and Quantification of Four Genetically Modified Soybean Events
作者：He, Yuxuan;Dong, Liming;Yan, Wei;Xing, Zhenjuan;Xia, Wei;Li, Congcong;Long, Likun;Li, Feiwu
关键词：Multiplex detection; Quantification; Digital PCR; GMO; Identification
-
Overexpression of the R2R3-MYB transcription factor GmMYB3a enhances isoflavone accumulation in soybean
作者：Xu, Zibo;Li, Jingwen;Zhang, Yongqiang;Wang, Ying;Zhu, Youcheng;Liu, Tianyi;Liu, Yajing;Wang, Qingyu;Yan, Fan;Song, Xue;He, Yuxuan
关键词：
-
Impact of different treatment methods and timings on soil microbial communities with transgenic maize straw return
作者：Xie, Yanbo;Long, Likun;Ma, Yue;Xing, Zhenjuan;Wang, Ling;Shao, Chunyu;Liu, Na;Li, Feiwu;Xiang, Jun-Yan
关键词：Soil microbiology; Genetically modified crops; Straw decomposition; Metagenomic sequencing; Agricultural sustainability
-
Amplification-free detection of genetically modified crops via spatial confinement CHA/HCR-aided surface-enhanced resonance Raman spectroscopy
作者：Bao, Chengxin;Wang, Huimin;Liu, Lin;Xu, Shuping;Li, Feiwu;He, Yuxuan;Yin, Yuejia;Liu, Xiangguo;Liu, Lin;Chang, Jingjing;Ding, Lan;Xu, Shuping;Xu, Shuping
关键词：Amplification-free; CHA; HCR; Spatial confinement; SERS
-
Development and collaborative validation of an event-specific quantitative real-time PCR method for detection of genetically modified CC-2 maize
作者：Long, Likun;Zhao, Ning;Li, Congcong;He, Yuxuan;Dong, Liming;Yan, Wei;Xing, Zhenjuan;Xia, Wei;Ma, Yue;Xie, Yanbo;Liu, Na;Li, Feiwu
关键词：genetically modified maize CC-2; event-specific PCR; quantification; real-time quantitative PCR; detection; validation
-
R2R3-MYB transcription factor GmMYB68 is involved in the accumulation of soybean isoflavones
作者：Xu, Zibo;Liu, Yajing;Zhao, Yuqian;Zhu, Youcheng;Wang, Ying;Li, Jingwen;Wang, Qingyu;Yan, Fan;Song, Xue;He, Yuxuan
关键词：Soybean isoflavones; GmMYB68; Gene regulation; Isoflavone biosynthesis
-
An effective droplet digital PCR method for identifying and quantifying meat adulteration in raw and processed food of beef (Bos taurus) and lamb (Ovis aries)
作者：He, Yuxuan;Yan, Wei;Dong, Liming;Ma, Yue;Li, Congcong;Xie, Yanbo;Liu, Na;Xing, Zhenjuan;Xia, Wei;Long, Likun;Li, Feiwu
关键词：food safety; quality control; droplet digital PCR; meat adulteration; beef; lamb
