Event-specific quantitative polymerase chain reaction methods for detection of double-herbicide-resistant genetically modified corn MON 87419 based on the 3 '-junction of the insertion site
文献类型： 外文期刊
作者： Long, Likun 1 ; Yan, Wei 1 ; Li, Congcong 1 ; Dong, Liming 1 ; Liu, Na 1 ; Xing, Zhenjuan 1 ; Li, Feiwu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun, Peoples R China
关键词： genetically modified corn; MON 87419; event-specific method; real-time PCR
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
ISSN： 0916-8451
年卷期： 2021 年 85 卷 6 期
页码：
收录情况： SCI
摘要： MON 87419 was one of the new transgenic corn events developed in US with the trait of herbicide resistance to both dicamba and glyphosate. To monitor unintended release of genetically modified organism in the future, as well as to meet GM-labeling requirements, it is requisite to develop a reliable method for the detection and quantification of MON 87419, an event-specific primer pair was designed to amplify the 3'-junction site between the endogenous genome sequence and the transferred DNA of GM event MON 87419, amplicons of desired size were produced by qualitative polymerase chain reaction (PCR) assay. For the validation of this quantitative method, the mixed samples containing 10%, 1%, and 0.1% MON 87419 ingredient were quantified. The precisions were expressed as relative standard deviations, deviated by 7.87%, 12.94%, and 19.98%, respectively. These results clearly demonstrate that the PCR methods we developed herein can be used for event-specific quantitative testing of the double-herbicide-resistant corn MON 87419.
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