Establishment and Application of Ligation Reaction-Based Method for Quantifying MicroR-156b
文献类型： 外文期刊
作者： He, Yuxuan 1 ; Long, Likun 1 ; Yan, Wei 1 ; Dong, Liming 1 ; Xia, Wei 1 ; Li, Congcong 1 ; Li, Feiwu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun, Peoples R China
关键词： MiRNAs; ribonucleotide-modified DNA probe; ligation reaction; quantitative method; MiR156b
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Microribonucleic acids (miRNAs) play significant roles in the regulation of biological processes and in responses to biotic or abiotic environmental stresses. Therefore, it is necessary to quantitatively detect miRNAs to understand these complicated biological regulation mechanisms. This study established an ultrasensitive and highly specific method for the quantitative detection of miRNAs using simple operations on the ground of the ligation reaction of ribonucleotide-modified deoxyribonucleic acid (DNA) probes. This method avoids the complex design of conventional reverse transcription. In the developed assay, the target miRNA miR156b was able to directly hybridize the two ribonucleotide-modified DNA probes, and amplification with universal primers was achieved following the ligation reaction. As a result, the target miRNA could be sensitively measured even at a detection limit as low as 0.0001 amol, and differences of only a single base could be detected between miR156 family members. Moreover, the proposed quantitative method demonstrated satisfactory results for overexpression-based genetically modified (GM) soybean. Ligation-based quantitative polymerase chain reaction (PCR) therefore has potential in investigating the biological functions of miRNAs, as well as in supervising activities regarding GM products or organisms.
- 相关文献
作者其他论文 更多>>
-
A Multiplex PCR System for the Detection and Quantification of Four Genetically Modified Soybean Events
作者：He, Yuxuan;Dong, Liming;Yan, Wei;Xing, Zhenjuan;Xia, Wei;Li, Congcong;Long, Likun;Li, Feiwu
关键词：Multiplex detection; Quantification; Digital PCR; GMO; Identification
-
Overexpression of the R2R3-MYB transcription factor GmMYB3a enhances isoflavone accumulation in soybean
作者：Xu, Zibo;Li, Jingwen;Zhang, Yongqiang;Wang, Ying;Zhu, Youcheng;Liu, Tianyi;Liu, Yajing;Wang, Qingyu;Yan, Fan;Song, Xue;He, Yuxuan
关键词：
-
Impact of different treatment methods and timings on soil microbial communities with transgenic maize straw return
作者：Xie, Yanbo;Long, Likun;Ma, Yue;Xing, Zhenjuan;Wang, Ling;Shao, Chunyu;Liu, Na;Li, Feiwu;Xiang, Jun-Yan
关键词：Soil microbiology; Genetically modified crops; Straw decomposition; Metagenomic sequencing; Agricultural sustainability
-
Amplification-free detection of genetically modified crops via spatial confinement CHA/HCR-aided surface-enhanced resonance Raman spectroscopy
作者：Bao, Chengxin;Wang, Huimin;Liu, Lin;Xu, Shuping;Li, Feiwu;He, Yuxuan;Yin, Yuejia;Liu, Xiangguo;Liu, Lin;Chang, Jingjing;Ding, Lan;Xu, Shuping;Xu, Shuping
关键词：Amplification-free; CHA; HCR; Spatial confinement; SERS
-
A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis
作者：Dong, Liming;Li, Congcong;Xing, Zhenjuan;He, Yuxuan;Xia, Wei;Long, Likun;Li, Feiwu;Liu, Yijun
关键词：Genetically modified soybean; High-throughput detection; Single universal primer multiplex PCR; Capillary electrophoresis
-
Development and collaborative validation of an event-specific quantitative real-time PCR method for detection of genetically modified CC-2 maize
作者：Long, Likun;Zhao, Ning;Li, Congcong;He, Yuxuan;Dong, Liming;Yan, Wei;Xing, Zhenjuan;Xia, Wei;Ma, Yue;Xie, Yanbo;Liu, Na;Li, Feiwu
关键词：genetically modified maize CC-2; event-specific PCR; quantification; real-time quantitative PCR; detection; validation
-
R2R3-MYB transcription factor GmMYB68 is involved in the accumulation of soybean isoflavones
作者：Xu, Zibo;Liu, Yajing;Zhao, Yuqian;Zhu, Youcheng;Wang, Ying;Li, Jingwen;Wang, Qingyu;Yan, Fan;Song, Xue;He, Yuxuan
关键词：Soybean isoflavones; GmMYB68; Gene regulation; Isoflavone biosynthesis
