Development and collaborative validation of an event-specific quantitative real-time PCR method for detection of genetically modified CC-2 maize
文献类型： 外文期刊
作者： Long, Likun 1 ; Zhao, Ning 1 ; Li, Congcong 1 ; He, Yuxuan 1 ; Dong, Liming 1 ; Yan, Wei 1 ; Xing, Zhenjuan 1 ; Xia, Wei 1 ; Ma, Yue 1 ; Xie, Yanbo 1 ; Liu, Na 1 ; Li, Feiwu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun, Peoples R China
关键词： genetically modified maize CC-2; event-specific PCR; quantification; real-time quantitative PCR; detection; validation
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： As one of the developed genetically modified (GM) maize varieties in China, CC-2 has demonstrated promising commercial prospects during demonstration planting. The establishment of detection methods is a technical prerequisite for effective supervision and regulation of CC-2 maize. In this study, we have developed an event-specific quantification method that targets the junction region between the exogenous gene and the 5' flanking genomic DNA (gDNA) of CC-2. The accuracy and precision of this method were evaluated across high, medium, and low levels of CC-2 maize content, revealing biases within +/- 25% and satisfactory precision data. Additionally, we determined the limits of quantification of the method to be 0.05% (equivalent to 20 copies) of the CC-2 maize. A collaborative trial further confirmed that our event-specific method for detecting CC-2 produces reliable, comparable, and reproducible results when applied to five different samples provided by various sources. Furthermore, we calculated the expanded uncertainty associated with determining the content level of CC-2 in these samples.
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