文献类型： 外文期刊
作者： Chao, Ouyang 1 ; Liu, Wei 2 ; Chen, Silan 1 ; Zhao, Huimin 1 ; Chen, Xinyan 1 ; Jin, Xiongxia 1 ; Li, Xinpeng 1 ; Wu, Yon 1 ;
作者机构： 1.Hainan Bolian Rice Gene Technol Co Ltd, Haikou, Hainan, Peoples R China
2.Guangdong Prov Key Lab New Technol Rice Breeding, Guangzhou, Peoples R China
关键词： glyphosate; EPSPS; goosegrass; rice; plant transformation
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）
ISSN： 1664-462X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Glyphosate-resistant crops developed by the CP4-EPSPS gene from Agrobacterium have been planted on a massive scale globally, which benefits from the high efficiency and broad spectrum of glyphosate in weed control. Some glyphosate-resistant (GR) genes from microbes have been reported, which might raise biosafety concerns. Most of them were obtained through a hygromycin-HPT transformation system. Here we reported the plant source with 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene from goosegrass endowed rice with high resistance to glyphosate. The integrations and inheritability of the transgenes in the rice genome were investigated within two generations. The EiEPSPS transgenic plants displayed similar growth and development to wild type under no glyphosate selection pressure but better reproductive performance under lower glyphosate selection pressure. Furthermore, we reconstructed a binary vector pCEiEPSPS and established the whole stage glyphosate selection using the vector. The Glyphosate-pCEiEPSPS selection system showed a significantly higher transformation efficiency compared with the hygromycin-HPT transformation system. Our results provided a promising alternative gene resource to the development of GR plants and also extended the plant transformation toolbox.
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