Nanoparticle vaccines based on the receptor binding domain of porcine deltacoronavirus elicit robust protective immune responses in mice
文献类型： 外文期刊
作者： Wang, Yuanhong 1 ; Song, Junhan 1 ; Deng, Xiaoying 1 ; Wang, Junna 1 ; Zhang, Miao 1 ; Liu, Yun 1 ; Tang, Pan 2 ; Liu, Huili 2 ; Zhou, Yanjun 1 ; Tong, Guangzhi 1 ; Li, Guoxin 1 ; Yu, Lingxue 1 ;
作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China
2.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai, Peoples R China
3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China
关键词： porcine deltacoronavirus; nanoparticle vaccine; ferritin; SpyTag/SpyCatcher; RBD
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.3； 五年影响因子：8.0 ）
ISSN： 1664-3224
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Background Porcine deltacoronavirus (PDCoV), a novel swine enteropathogenic coronavirus, challenges the global swine industry. Currently, there are no approaches preventing swine from PDCoV infection.Methods A new PDCoV strain named JS2211 was isolated. Next, the dimer receptor binding domain of PDCoV spike protein (RBD-dimer) was expressed using the prokaryotic expression system, and a novel nanoparticle containing RBD-dimer and ferritin (SC-Fe) was constructed using the SpyTag/SpyCatcher system. Finally, the immunoprotection of RBD-Fe nanoparticles was evaluated in mice.Results The novel PDCoV strain was located in the clade of the late Chinese isolate strains and close to the United States strains. The RBD-Fe nanoparticles were successfully established. Immune responses of the homologous prime-boost regime showed that RBD-Fe nanoparticles efficiently elicited specific humoral and cellular immune responses in mice. Notably, high level PDCoV RBD-specific IgG and neutralizing antibody (NA) could be detected, and the histopathological results showed that PDCoV infection was dramatically reduced in mice immunized with RBD-Fe nanoparticles.Conclusion This study effectively developed a candidate nanoparticle with receptor binding domain of PDCoV spike protein that offers protection against PDCoV infection in mice.
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