您好，欢迎访问天津市农业科学院 机构知识库！
天津市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A novel recombinant adenovirus expressing apoptin and melittin genes kills hepatocellular carcinoma cells and inhibits the growth of ectopic tumor

文献类型： 外文期刊

作者： Wu, Jingqiao 1 ; Lan, Zhaoyu 1 ; Li, Xin 1 ; He, Jinling 1 ; Zhang, Dongchao 1 ; Jin, Tianming 1 ;

作者机构： 1.Tianjin Agr Univ, Minist Agr & Rural Affairs, Key Lab Smart Breeding Coconstruct Minist & Prov, Tianjin 300392, Peoples R China

2.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China

3.Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Key Lab Agr Anim Breeding & Hlth Husb, Tianjin 300392, Peoples R China

4.Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Engn Technol Ctr Livestock Pathogen Detect, Tianjin 300392, Peoples R China

关键词： Recombinant adenovirus; Melittin; Apoptin; Hepatocellular carcinoma

期刊名称：INVESTIGATIONAL NEW DRUGS （ 影响因子：3.0； 五年影响因子：2.9 ）

ISSN： 0167-6997

年卷期： 2024 年 42 卷 4 期

页码：

收录情况： SCI

摘要： HCC is the most common fatal malignancy. Although surgical resection is the primary treatment strategy, most patients are not eligible for resection due to tumor heterogeneity, underlying liver disease, or comorbidities. Therefore, this study explores the possibility of multi-molecular targeted drug delivery in treating HCC. In this study, we constructed the recombinant adenovirus co-expressing apoptin and melittin (MEL) genes. The inhibitory effect of the recombinant adenovirus on hepatocellular carcinoma cells was detected through experiments on cell apoptosis, migration, invasion, and other factors. The tumor inhibitory effect in vivo was assessed using subcutaneous HCC mice. Results showed that recombinant adenovirus co-expressing anti-tumor genes TAT and apoptin, RGD and MEL can significantly inhibit the proliferation, migration, and invasion of HCC cells by inducing an increase in reactive oxygen species (ROS) levels, upregulation of apoptotic proteins such as Bax, cleaved caspase-3, and cleaved caspase-9, and downregulation of the anti-apoptotic protein Bcl-2. In subcutaneous HCC mice, recombinant adenovirus induced significant apoptosis in tumor, and inhibited tumor growth. In conclusion, recombinant adenovirus co-expressing apoptin and MEL can inhibit the growth and proliferation of tumor cells both in vivo and in vitro.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：天津市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台