文献类型： 外文期刊

作者： Chen, Zhiyin ¹ ; Liu, Baogui ¹ ; Gong, Zhihua ¹ ; Huang, Hua ⁶ ; Gong, Yihui ² ; Xiao, Wenjun ¹ ;

作者机构： 1.Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Peoples R China

2.Hunan Univ Humanities Sci & Technol, Coll Agr & Biotechnol, Loudi 417000, Peoples R China

3.Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China

4.Hunan Agr Univ, Educ Minist Utilizat Bot Funct Ingredients, Coinnovat Ctr, Changsha 410128, Peoples R China

5.Minist Agr & Rural Affairs China, Key Lab Evaluat & Utilizat Gene Resources Hort Cr, Changsha 410128, Peoples R China

6.Guangdong Acad Agr Sci, Key Lab South Subtrop Fruit Biol & Genet Resource, Minis Try Agr & Rural Affairs,Inst Fruit Tree Re, Guang Dong Prov Key Lab Trop & Subtrop Fruit Tree, Guangzhou 510640, Peoples R China

关键词： (-)-epigallocatechin-3-gallate; nanoparticles; intestinal microbiome; hyperlipidemia; metagenomics

期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）

ISSN：

年卷期： 2022 年 27 卷 15 期

页码：

收录情况： SCI

摘要： The effects of nanoparticles (NPs) on microbiota homeostasis and their physiological relevance are still unclear. Herein, we compared the modulation and consequent pharmacological effects of oral administration of (-)-epigallocatechin-3-gallate (EGCG)-loaded beta-cyclodextrin (beta-CD) NPs (EGCG@beta-CD NPs) and EGCG on gut microbiota. EGCG@beta-CD NPs were prepared using self-assembly and their influence on the intestinal microbiome structure was analyzed using a metagenomics approach. The "Encapsulation efficiency (EE), particle size, polydispersity index (PDI), zeta potential" of EGCG@beta-CD NPs were recorded as 98.27 +/- 0.36%, 124.6 nm, 0.313 and -24.3 mV, respectively. Surface morphology of EGCG@beta-CD NPs was observed as spherical. Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and molecular docking studies confirmed that EGCG could be well encapsulated in beta-CD and formed as EGCG@beta-CD NPs. After being continuously administered EGCG@beta-CD NPs for 8 weeks, the serum cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and liver malondialdehyde (MDA) levels in the rats were significantly decreased, while the levels of catalase (CAT) and apolipoprotein-A1 (apo-A1) in the liver increased significantly in the hyperlipidemia model of rats, when compared to the high-fat-diet group. Furthermore, metagenomic analysis revealed that the ratio of Verrucomicrobia/Bacteroidetes was altered and Bacteroidetes decreased in the high-fat diet +200 mg/kg center dot bw EGCG@beta-CD NPs group, while the abundance of Verrucomicrobia was significantly increased, especially Akkermansia muciniphila in rat feces. EGCG@beta-CD NPs could be a promising EGCG delivery strategy to modulate the gut microbiota, enhancing its employment in the prevention of hyperlipidemia.