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Microfluidics: the propellant of CRISPR-based nucleic acid detection

文献类型： 外文期刊

作者： Chen, Yanju 1 ; Qian, Siwenjie 1 ; Yu, Xiaoping 3 ; Wu, Jian 1 ; Xu, Junfeng 2 ;

作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China

2.Zhejiang Acad Agr Sci, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China

3.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China

4.ZJU Hangzhou Global Sci & Technol Innovat Ctr, 733 Jianshe 3rd Rd, Hangzhou 311200, Peoples R China

期刊名称：TRENDS IN BIOTECHNOLOGY （ 影响因子：17.3； 五年影响因子：18.1 ）

ISSN： 0167-7799

年卷期： 2023 年 41 卷 4 期

页码：

收录情况： SCI

摘要： Since the discovery of collateral cleavage activity, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems have become the new generation of nucleic acid detection tools. However, their widespread application remains limited. A pre-amplification step is required to improve the sensitivity of CRISPR systems, complicating the operating procedure and limiting quantitative precision. In addition, nonspecific collateral cleavage activity makes it difficult to realize multiplex detection in a one-pot CRISPR reaction with a single Cas protein. Microfluidics, which can transfer nucleic acid analysis process to a chip, has the advantages of miniaturization, integration, and automation. Microfluidics coupled with CRISPR systems improves the detection ability of CRISPR, enabling fast, high-throughput, integrated, multiplex, and digital detection, which results in the further popularization of CRISPR for a range of scenarios.

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