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LAMP-visualized photofuel cell self-powered dual-mode sensing platform for detection of transmissible gastroenteritis virus

文献类型： 外文期刊

作者： Yuan, Ruishuang 1 ; Ma, Hanyu 2 ; Min, Yinmin 2 ; Ding, Lijun 1 ; Li, Bin 3 ; Wang, Kun 1 ;

作者机构： 1.Jiangsu Univ, Sch Agr Engn, Key Lab Modern Agr Equipment & Technol, Minist Educ, Zhenjiang 212013, Jiangsu, Peoples R China

2.Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

关键词： Electrochromic visualization; Photocatalytic fuel cells; Loop-mediated isothermal amplification; Transmissible gastroenteritis virus; CdS/ZnO NRs

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）

ISSN： 0039-9140

年卷期： 2024 年 274 卷

页码：

收录情况： SCI

摘要： The detection of transmissible gastroenteritis virus (TGEV) is of great significance to reduce the loss of pig industry. A LAMP-visualization/PFC self-powered dual-mode output sensor platform was constructed to detect TGEV by combining a simple and intuitive photoelectrochromic material with a highly sensitive PFC selfpowered sensing platform without external power supply. The PFC sensing substrate was constructed using CdS nanoparticles modified ZnO NRs (CdS/ZnO NRs) as the photoanode, which exhibited high photoactivity, and Prussian blue (PB) as the cathode. After LAMP reaction on the optical anode, visual signals caused by PB discolorimetry can be detected semi-quantitatively, or PFC power density electrical signals collected by electrochemical workstation can be used. The output power density value is logarithm of TGEV concentration. The linear relationship was good within the detection range of 0.075 fg/mu L-7.5 ng/mu L, with a detection limit of 0.025 fg/mu L (S/N = 3). This multi-signal output sensing platform provides more choices for quantifying TGEV detection results, and the two methods can be mutually verified, which meets the needs of different scenarios and improves the reliability of detection. It has a good effect in the actual sample detection, without the use of expensive and complex instruments, and has a broad application prospect.

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