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Molecular target for sprayable double-stranded RNA-based biopesticide against Amphitetranychus viennensis (Acari, Tetranychidae)

文献类型： 外文期刊

作者： Duan, Yuanpeng 1 ; Wang, Yifei 1 ; Yang, Fan 1 ; Gao, Yue 1 ; Liu, Zhongfang 1 ; Zhang, Pengjiu 1 ; Lu, Junjiao 1 ; Fan, Renjun 1 ; Zhou, Xuguo 2 ; Yang, Jing 1 ; Ren, Meifeng 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Plant Protect, Shanxi Key Lab Integrated Pest Management Agr, Taiyuan 030031, Shanxi, Peoples R China

2.Univ Illinois, Coll Liberal Arts & Sci, Sch Integrat Biol, Dept Entomol, Urbana, IL 61801 USA

关键词： Target gene screening; Sprayable dsRNA-based biopesticide; Spray-induced gene silencing; Proteasome subunit beta 5; Spider mites; Amphitetranychus viennensis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 289 卷

页码：

收录情况： SCI

摘要： Amphitetranychus viennensis, a destructive pest mite of fruit plants in Europe and Asia, poses a serious challenge due to its adaptability and resistance to multiple acaricides. RNA interference (RNAi)-based technologies offer a promising alternative to address this emerging issue. In this study, we screened for candidate genes that can be targeted for spray-induced gene silencing (SIGS). Suppression of AvSrp54k, AveIF4A-1, AvHel31B, AvCOPB2 and AvProsbeta5 led to a significantly higher mortality and caused minor damages to leaf discs in comparison to the controls. Among them, AvCOPB2 and AvProsbeta5 were the best candidates with the highest mortality (>95 %) and minimal leaf damages (<13 %). Given that LdProsbeta5 is the active ingredient of the first sprayable dsRNAbased biopesticide, Ledprona, against the Colorado Potato Beetle, Leptinotarsa decemlineata, we examined the suitability of AvProsbeta5 in managing A. viennensis. In comparison to the control, A. viennensis population was suppressed by >95% at day-17, and the plant defoliation rate decreased to 0 at day-24. Our combined results not only provide two viable molecular targets for sprayable dsRNA-based biopesticides, but also confirm the practical implications of SIGS in managing A. viennensis, one of the most destructive arthropod pests in orchards and ornamental plants.

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