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The development of an egg-soaking method for delivering dsRNAs into spider mites

文献类型： 外文期刊

作者： Yang, Jing 1 ; Zhang, Yuying 1 ; Zhang, Zhonghuan 1 ; Ren, Meifeng 1 ; Wang, Yifei 1 ; Duan, Yuanpeng 1 ; Gao, Yue 1 ; Liu, Zhongfang 1 ; Zhang, Pengjiu 1 ; Fan, Renjun 1 ; Zhou, Xuguo 2 ;

作者机构： 1.Shanxi Agr Univ, Coll Plant Protect, Shanxi Key Lab Integrated Pest Management Agr, Taiyuan, Shanxi, Peoples R China

2.Univ Illinois, Coll Liberal Arts & Sci, Sch Integrat Biol, Dept Entomol, Urbana, IL 61801 USA

关键词： dsRNA delivery; Egg-soaking RNAi method; Sprayable RNAi biopesticide; Spider mites; Amphitetranychus viennensis; Tetranychus urticae

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2024 年 201 卷

页码：

收录情况： SCI

摘要： Recently, the first sprayable RNAi biopesticide, Ledprona, against the Colorado potato beetle, Leptinotarsa decemlineata, has been registered at the United States Environmental Protection Agency. Spider mites (Acari: Tetranychidae), a group of destructive agricultural and horticultural pests, are notorious for rapid development of insecticide/acaricide resistance. The management options, on the other hand, are extremely limited. RNAibased biopesticides offer a promising control alternative to address this emerging issue. In this study, we i) developed an egg-soaking dsRNA delivery method; ii) evaluated the factors influencing RNAi efficiency, and finally iii) investigated the potential mode of entry of this newly developed egg-soaking RNAi method. In comparison to other dsRNA delivery methods, egg-soaking method was the most efficient, convenient/practical, and cost-effective method for delivering dsRNAs into spider mites. RNAi efficiency of this RNAi method was affected by target genes, dsRNA concentration, developmental stages, and mite species. In general, the hawthorn spider mite, Amphitetranychus viennensis, is more sensitive to RNAi than the two-spotted spider mite, Tetranychus urticae, and both of them have dose-dependent RNAi effect. For different life stages, egg and larvae are the most sensitive life stages to dsRNAs. For different target genes, there is no apparent association between the suppression level and the resultant phenotype. Finally, we demonstrated that this egg-soaking RNAi method acts as both stomach and contact toxicity. Our combined results demonstrate the effectiveness of a topically applied dsRNA delivery method, and the potential of a spray induced gene silencing (SIGS) method as a control alternative for spider mites.

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