Transposon-tagging identifies novel pathogenicity genes in Fusarium graminearum
文献类型： 外文期刊
作者： Dufresne, Marie 1 ; van der Lee, Theo 2 ; Ben M'Barek, Sarrah 1 ; Xu, Xiude 2 ; Zhang, Xu 2 ; Liu, Taiguo 1 ; Waalwijk 1 ;
作者机构： 1.Univ Paris 11, CNRS, UMR8621, Inst Genet & Microbiol, F-91405 Orsay, France
2.Plant Res Int BV, NL-6500 AA Wageningen, Netherlands
3.Liaoning Acad Agr Sci, Inst Plant Protect, Shenyang 110161, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Genet, Nanjing 210014, Jiangsu, Peoples R China
5.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China
6.Chinese Acad Agr Sci, Inst Plant Protect,
期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.495； 五年影响因子：3.831 ）
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收录情况： SCI
摘要： With the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7%) with altered phenotypes. Complementation with the original gene restored the wild-type phenotype in two selected mutants demonstrating the high tagging efficiency. This is the first report of a MITE transposon tagging system as an efficient mutagenesis tool in F. graminearum.
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