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Application of green deep eutectic solvents for anthocyanins extraction from grape pomace: Optimization, stability, antioxidant activity, and molecular dynamic simulation

文献类型: 外文期刊

作者: Li, Zhongxu 1 ; Cui, Ruiguo 1 ; Liu, Weiwei 2 ; Wang, Mengshi 2 ; Li, Lilang 5 ; Liu, Fengjuan 3 ; Du, Bin 2 ; Song, Lijun 2 ;

作者机构: 1.Hebei Normal Univ Sci & Technol, Coll Food Sci & Technol, Qinghai 066600, Hebei, Peoples R China

2.Hebei Normal Univ Sci & Technol, Chestnut Res Ctr, Qinhuangdao 066004, Hebei, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Qual Stand & Testing Technol Agriprod, Urumqi 830091, Peoples R China

4.Hebei Normal Univ Sci & Technol, Hebei Key Lab Nat Prod Act Components & Funct, Qinhuangdao 066004, Hebei, Peoples R China

5.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China

关键词: Grape pomace; Anthocyanins; Deep eutectic solvent; Stability; Molecular dynamics simulation

期刊名称:LWT-FOOD SCIENCE AND TECHNOLOGY ( 影响因子:6.6; 五年影响因子:6.9 )

ISSN: 0023-6438

年卷期: 2024 年 211 卷

页码:

收录情况: SCI

摘要: The wine-making industry produces a large amount of grape pomace, which is rich in anthocyanins. To avoid wasting these resources, choline chloride: lactic acid (molar ratio of 1:2) (DES-1) was selected as the optimal solvent. The optimal extraction conditions were as follows: temperature 60 degrees C, time 60 min, water content 25%. The anthocyanins yield was 5.73 mg (cyanidin-3-glucoside) equivalent/g under the optimal conditions, which was significantly higher than that obtained by DES-3 (Choline chloride: 1,4 butanediol), DES-5 (Choline chloride: 1,2 propylene glycol), and ethanol. The DES-1 extract showed significant higher color stability and antioxidant activities compared with the anthocyanins extracted by DES-3, DES-5, and ethanol. A total of eight individual anthocyanin compounds were identified in each extract, with delphinidin 3-O-glucoside, peonidin 3-O- glucoside, petunidin, and delphinidin as the dominant compounds. Molecular dynamics simulation confirmed that DES-1 system showed a larger average number of hydrogen bonds (11.63), average lifetime of hydrogen bonds (364.21 ps) and with a reduced total interaction energy (-706.82 kJ/moL), thus improving the extraction efficiency and stability of anthocyanins. This study would provide new protocols for the extraction of anthocyanins from grape pomace.

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