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Growth Axis Somatostatin, Growth Hormone Receptor, and Insulin-like Growth Factor-1 Genes Express and Are Affected by the Injection of Exogenous Growth Hormone in Chinemys reevesii

文献类型： 外文期刊

作者： Xie, Rui-Lin 1 ; Liang, Rui 2 ; Luo, Yuan-Yuan 1 ; Ruan, Zhuo-Hao 3 ; Li, Yi-Fu 1 ; Liu, Wen-Sheng 1 ;

作者机构： 1.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Peoples R China

2.Foshan Inst Agr Sci, Foshan 528251, Peoples R China

3.Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed Sci South China, Guangdong Key Lab Anim Breeding & Nutr, Minist Agr Rural Affairs,Inst Anim Sci,Lab Aquat S, Guangzhou 510640, Peoples R China

4.South China Agr Univ, Guangdong Prov Engn Res Ctr Aquat Immunizat & Aqua, Guangzhou 510642, Peoples R China

5.Univ Joint Lab Guangdong Prov Hong Kong & Macao Re, Guangzhou 510642, Peoples R China

关键词： Chinemys reevesii; growth hormone injection; somatostatin; growth hormone receptor; insulin-like growth factor-I

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 11 期

页码：

收录情况： SCI

摘要： In this study, to explore the effect of growth hormone changes on the related genes and regulatory roles of the turtle, PCR amplification, real-time fluorescence quantitative analysis, and enzyme cutting technology were used to clone and sequence the somatostatin (SS) gene, growth hormone receptor (GHR), and insulin-like growth factor-1 (IGF-I) sequence of Chinemys reevesii. The effects of human growth hormone on the mRNA expression of growth-axis-related genes SS, GHR, and IGF-1 in different sexes were observed. The study of the SS gene in turtles using real-time fluorescence quantitative PCR showed that the SS gene was mainly expressed in the nervous system and the digestive system, with the highest expression found in the brain, while the GHR gene and the IGF-I gene were expressed in all tissues of Chinemys reevesii. The SS gene was expressed in the brain, pituitary, liver, stomach, and intestine, with the highest expression in the brain and the lowest expression in the liver. Within 4 weeks of the injection of exogenous growth hormone, the expression level of the SS gene in the brain of both sexes first increased and then decreased, showing a parabolic trend, and the expression level of the experimental group was lower than that of the control group. After the injection of growth hormone (GH), the expression of the GHR gene in the liver of both sexes showed a significant increase in the first week, decreasing to the control group level in the second week, and then gradually increasing. Finally, a significant level of difference in the expression of the GHR gene was reached at 3 and 4 weeks. In terms of the IGF-I gene, the changing trend of the expression level in the liver was the same as that of the GHR gene. After the injection of exogenous growth hormone, although the expression of the SS gene increased the inhibition of the secretion of the GHR gene by the Reeves' turtle, exogenous growth hormone could replace the synthesis of GH and GHR, accelerating the growth of the turtle. The experiments showed that the injection of recombinant human growth hormone affects the expression of SS, GHR, and IGF-1 genes, and promotes the growth of the Reeves' turtle.

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