您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Novel SNPs in the 3 ' UTR Region of GHRb Gene Associated with Growth Traits in Striped Catfish (Pangasianodon hypophthalmus), a Valuable Aquaculture Species

文献类型： 外文期刊

作者： Jiang, Liang-Sen 1 ; Ruan, Zhuo-Hao 1 ; Lu, Zhi-Qiang 1 ; Li, Yi-Fu 1 ; Luo, Yuan-Yuan 1 ; Zhang, Xi-Quan 4 ; Liu, Wen-Sheng 1 ;

作者机构： 1.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Sci,Coll Marine Sci, Lab Aquat Sci,Key Lab Anim Nutr & Feed Sci South, Guangdong Key Lab Anim Breeding & Nutr,Minist Agr, Guangzhou 510640, Peoples R China

3.South China Agr Univ, Guangdong Prov Engn Res Ctr Aquat Immunizat & Aqu, Guangzhou 510642, Peoples R China

4.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China

5.Univ Joint Lab Guangdong Prov, Hong Kong & Macao Reg Marine Bioresource Conserva, Guangzhou 510642, Peoples R China

关键词： Pangasianodon hypophthalmus; striped catfish; GHRb; association analysis; growth traits; markers for selective breeding; qRT-PCR

期刊名称：FISHES （ 影响因子：3.17； 五年影响因子：3.098 ）

ISSN：

年卷期： 2022 年 7 卷 5 期

页码：

收录情况： SCI

摘要： The striped catfish, Pangasianodon hypophthalmus is an important freshwater aquaculture species in Southeast Asian countries due to its rapid growth. The growth hormone receptor (GHR) is a significant regulatory factor for the growth axis and has great potential applications in animal genetic breeding. This study aims to characterize the GHRb cDNA of the striped catfish and analyze the distribution of its mRNA. Screening of single nucleotide polymorphisms' (SNPs) loci and diplotypes was performed to provide basic information for the assisted selection of molecular markers in genetic breeding. The results showed that the GHRb cDNA of striped catfish had 2791 bp, which encoded for 569 amino acids. In a phylogenyic study, the ghrb of the striped catfish was clustered with those of other catfish and they were highly homologous. Quantitative real-time PCR (qRT-PCR) experiments showed that GHRb mRNA was expressed in ten different organs of the striped catfish, with the highest expression level in the liver. Five SNP and a haplotype block were identified in the 3 ' UTR of the GHRb gene using the direct sequencing of 307 individuals. Three haplotypes were found and four diplotypes were constructed. The association analysis revealed that these polymorphisms were significantly associated with growth traits in the striped catfish (p < 0.05). These polymorphisms will provide a valuable reference for future molecular genetic marker-assisted breeding of striped catfish.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台