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In Vitro Antifungal Activity of Dihydrochelerythrine and Proteomic Analysis in Ustilaginoidea virens

文献类型： 外文期刊

作者： Wei, Qinghui 1 ; Cui, Daizong 1 ; Zheng, Baojiang 1 ; Zhao, Min 1 ;

作者机构： 1.Northeast Forestry Univ, Harbin 150040, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Plant Protect, Harbin 150086, Peoples R China

关键词： dihydrochelerythrine; Ustilaginoidea virens; antifungal activity; spore growth; apoptosis

期刊名称：RICE SCIENCE （ 影响因子：4.8； 五年影响因子：5.5 ）

ISSN： 1672-6308

年卷期： 2023 年 30 卷 3 期

页码：

收录情况： SCI

摘要： Dihydrochelerythrine (DHCHE) is an isoquinoline compound, which has distinct antifungal activity and can induce apoptosis. The antifungal activity of DHCHE against five rice pathogenic fungi was studied in vitro. At the concentration of 7.5 mg/L, DHCHE exhibited the highest efficacy among tested compounds in inhibiting mycelium growth, with an inhibition rate of 68.8% in Ustilaginoidea virens, which was approximately 2.4 times of that of validamycin (28.7%). After exposure to DHCHE, transmission electron micrographs revealed spores showed incomplete organelles, malformed cell walls and nuclear membranes, as well as irregular lipid spheres. Reactive oxygen species accumulation in treated spores was markedly higher than that in control spores. DHCHE induced cell damage increased in a dose-dependent manner, as indicated by the decrease in mitochondrial membrane potential and initiation of apoptosis. The differences of expression levels of Fip1, ACP1, PMS2 and COX13 that are important for oxidative phosphorylation and mismatch repair pathway were significant, which may be some of the reasons for the induction of apoptosis in DHCHE-treated U. virens. The protein levels of Fip1, ACP1, PMS2 and COX13 agreed with protein fold change ratio from parallel reaction monitoring Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathway of differentially expressed proteins were further analyzed. These findings will help to elucidate the mechanisms associated with antifungal and pro-apoptotic effects of DHCHE on U. virens, thereby aiding the potential development of novel pesticides.

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