文献类型： 外文期刊

作者： Wei, Qing-Hui ¹ ; Liu, Xue-Feng ¹ ; Chai, Yang-Yang ¹ ; Liang, Ming ¹ ; Zheng, Bao-Jiang ¹ ; Zhao, Min ¹ ;

作者机构： 1.Northeast Forestry Univ, 26, Hexing Rd, Harbin 150040, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Plant Protect, 368, Xuefu Rd, Harbin 150086, Peoples R China

关键词： Chelerythrine; Ustilaginoidea virens; Antifungal activity; Apoptosis; Bioinformatics; TMT-based quantitative proteomics

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）

ISSN： 0929-1873

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Chelerythrine (CHE) is a natural benzophenanthridine alkaloid with broad pharmacological activities. Herein, we investigated the responses to CHE of the fungal pathogen Ustilaginoidea virens using a proteomics approach. At 7.5 x 10(-3) mg/mL, the U. virens inhibition rate reached 56.1%, significantly more potent than another two isoquinoline alkaloids and the commercial fungicide validamycin. The disease control effect on plants in the field was > 50% after spraying with 500 x liquid containing 20% CHE, significantly better than that of validamycin. A total of 4734 proteins were identified in CHE-treated U. virens using tandem mass tag (TMT)-based quantitative proteomics, and 823 were differentially expressed proteins (DEPs). Gene Ontology (GO) of DEPs yielded 107 biological process (BP) subcategories, 45 cellular component (CC) subcategories and 43 molecular function (MF) subcategories. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were employed, and seven proteins were selected for subsequent parallel reaction monitoring (PRM) analysis. The results indicate that CHE might lead to apoptosis, but further studies are necessary.