文献类型： 外文期刊

作者： Zhang, Jianmin ¹ ; Peng, Zhong ⁵ ; Chen, Kaifeng ¹ ; Zhan, Zeqiang ¹ ; Shen, Haiyan ⁸ ; Feng, Saixiang ¹ ; Gou, Hongchao ⁸ ; Qu, Xiaoyun ¹ ; Ziemann, Mark ⁹ ; Layton, Daniel S. ¹⁰ ; Wang, Xiangru ⁵ ; Chen, Huanchun ⁵ ; Wu, Bin ⁵ ; Xu, Xuebin ¹¹ ; Liao, Ming ¹ ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Natl & Reg Joint Engn Lab Medicament Zoonoses Prev, Guangzhou, Peoples R China

2.South China Agr Univ, Coll Vet Med, Key Lab Zoonoses, Minist Agr, Guangzhou, Peoples R China

3.South China Agr Univ, Coll Vet Med, Key Lab Zoonoses Prevent & Control Guangdong Prov, Guangzhou, Peoples R China

4.South China Agr Univ, Coll Vet Med, Anim Infect Dis Lab, Guangzhou, Peoples R China

5.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Peoples R China

6.Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Peoples R China

7.Hubei Hongshan Lab, Wuhan, Peoples R China

8.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China

9.Deakin Univ, Sch Life & Environm Sci, Waurn Ponds Campus, Geelong, Vic, Australia

10.Australian Ctr Dis Prevent, Commonwealth Sci & Ind Res Org Hlth & Biosecur, East Geelong, Vic, Australia

11.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai, Peoples R China

关键词： IncFII(S) type plasmid; ST365; Salmonella Weltevreden; human diarrhea; whole-genome sequence and phylogenetic analysis

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Salmonella Weltevreden is a pathogen associated with human diarrheal diseases found across the globe. However, knowledge of the genomics and epidemiology of this pathogen is still limited. Salmonella Weltevreden is an emerging pathogen associated with human diarrhea, and knowledge of the genomics and epidemiology of this serovar is still limited. In this study, we performed whole-genome sequencing of 96 S. Weltevreden isolates recovered from diarrheal patients and 62 isolates from food animals in China between 2006 and 2017. Together, with an additional 199 genome sequences of S. Weltevreden published in NCBI, we performed an analysis on all 357 S. Weltevreden genome sequences. Our results demonstrated that the majority of S. Weltevreden from diarrheal patients from China (97.92%, 94/96) and the other regions in the world (94.97%, 189/199) identified in this study were sequence type (ST) 365. The remaining types were ST3771 (n = 3), ST22 (n = 1), ST155 (n = 1), and ST684 (n = 1). In addition, ST365 was also widely recovered from animals, food, and environmental samples in different regions of the world. Phylogenetic analysis and pulsed-field gel electrophoresis (PFGE) revealed that S. Weltevreden from diarrheal patients was closely related to those recovered from food and environmental specimens. We also showed that S. Weltevreden did not exhibit severe antimicrobial resistance profiles, suggesting administering antibiotics is still effective for controlling the agent. Interestingly, we found that S. Weltevreden strains carried a number of virulence factor genes, and a 100.03-kb IncFII(S) type plasmid was widely distributed in S. Weltevreden strains. Elimination of this plasmid decreased the bacterial capacity to infect both Caco-2 cells and C57BL/6 mice, suggesting the importance of this plasmid for bacterial virulence. Our results contribute to the understanding of the epidemiology and virulence of S. Weltevreden.IMPORTANCE Salmonella Weltevreden is a pathogen associated with human diarrheal diseases found across the globe. However, knowledge of the genomics and epidemiology of this pathogen is still limited. In this study, we found S. Weltevreden sequence type (ST) 365 is commonly recovered from diarrheal patients in China and many other regions of the world, and there is no major difference between the Chinese isolates and the global isolates at the phylogenetic level. We also demonstrated that ST365 was widely recovered from animal, food, and environmental samples collected in different, global regions. Importantly, we discovered an IncFII(S) type plasmid commonly carried by S. Weltevreden strains of human, animal, and food origins, and this plasmid is likely to contribute to the bacterial pathogenesis. These findings enhance our understanding of the emergence of S. Weltevreden involved in diarrheal outbreaks and the global spread of S. Weltevreden strains.