文献类型： 外文期刊

作者： Hulst, Marcel ¹ ; Kant, Arie ¹ ; Harders-Westerveen, Jose ¹ ; Hoffmann, Markus ² ; Xie, Yajing ⁴ ; Laheij, Charlotte ⁵ ; Murk, Jean-Luc ⁶ ; Van der Poel, Wim H. M. ¹ ;

作者机构： 1.Wageningen Biovet Res, Dept Virol & Mol Biol, NL-8221 RA Lelystad, Netherlands

2.Leibniz Inst Primate Res, German Primate Ctr, Infect Biol Unit, D-37077 Gottingen, Germany

3.Univ Gottingen, Fac Biol & Psychol, D-37073 Gottingen, Germany

4.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China

5.Elisabeth Tweesteden Hosp, NL-5022 GC Tilburg, Netherlands

6.Elisabeth Tweesteden Hosp, Microvida, NL-5022 GC Tilburg, Netherlands

关键词： SARS-CoV-2; alpha- and beta-coronaviruses; cross-neutralization; pre- and post-pandemic sera; humans; farm animals; wild boars

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Panels of pre- and post-pandemic farm animals, wild boar and human sera, including human sera able to neutralize SARS-CoV-2 in vitro, were tested in serological tests to determine their cross-reactivity with beta- and alpha-CoV originating from farm animals. Sera were tested in neutralization assays with high ascending concentrations (up to 1 x 10(4) TCID50 units/well) of beta-CoV Bovine coronavirus (BCV), SARS-CoV-2, and porcine alpha-CoV-transmissible gastroenteritis virus (TGEV). In addition, sera were tested for immunostaining of cells infected with beta-CoV porcine hemagglutinating encephalomyelitis (PHEV). Testing revealed a significantly higher percentage of BCV neutralization (78%) for sera of humans that had experienced a SARS-CoV-2 infection (SARS-CoV-2 convalescent sera) than was observed for human pre-pandemic sera (37%). Also, 46% of these human SARS-CoV-2 convalescent sera neutralized the highest concentration of BCV (5 x 10(3) TCID50/well) tested, whereas only 9.6% of the pre-pandemic sera did. Largely similar percentages were observed for staining of PHEV-infected cells by these panels of human sera. Furthermore, post-pandemic sera collected from wild boars living near a densely populated area in The Netherlands also showed a higher percentage (43%) and stronger BCV neutralization than was observed for pre-pandemic sera from this area (21%) and for pre- (28%) and post-pandemic (20%) sera collected from wild boars living in a nature reserve park with limited access for the public. High percentages of BCV neutralization were observed for pre- and post-pandemic sera of cows (100%), pigs (up to 45%), sheep (36%) and rabbits (60%). However, this cross-neutralization was restricted to sera collected from specific herds or farms. TGEV was neutralized only by sera of pigs (68%) and a few wild boar sera (4.6%). None of the BCV and PHEV cross-reacting human pre-pandemic, wild boar and farm animal sera effectively neutralized SARS-CoV-2 in vitro. Preexisting antibodies in human sera effectively neutralized the animal beta-CoV BCV in vitro. This cross-neutralization was boosted after humans had experienced a SARS-CoV-2 infection, indicating that SARS-CoV-2 activated a "memory" antibody response against structurally related epitopes expressed on the surface of a broad range of heterologous CoV, including beta-CoV isolated from farm animals. Further research is needed to elucidate if a symptomless infection or environmental exposure to SARS-CoV-2 or another beta-CoV also triggers such a "memory" antibody response in wild boars and other free-living animals.