Mulberry leaf extract reduces damage by regulating the oxidative response, immune response and intestinal flora of largemouth bass (Micropterus salmoides) under chronic heat stress
文献类型： 外文期刊
作者： Fu, Bing 1 ; Zhou, Donglai 1 ; Qiu, Xiaotong 1 ; Zheng, Jieqiu 1 ; Yang, Qiong 1 ; Xiao, Yang 1 ; Liao, Sentai 1 ; Li, Qingrong 1 ; Xing, Dongxu 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc, Minist Agr & Rural Affairs,Sericultural & Agrifood, Guangzhou, Peoples R China
2.South China Agr Univ, Coll Marine Sci, Guangzhou, Peoples R China
3.Zhongkai Univ Agr & Engn, Coll Food Sci & Technol, Guangzhou, Peoples R China
关键词： mulberry leaf extract; largemouth bass; heat stress; intestinal flora; liver injury; immune ability
期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：3.0； 五年影响因子：3.8 ）
ISSN：
年卷期： 2025 年 12 卷
页码：
收录情况： SCI
摘要： Chronic heat stress (HS) induces oxidative damage, low immunity, and intestinal flora disturbance of fish, posing great challenges to the aquaculture industry. As an important plant extract, mulberry leaf extract (MLE) has been shown to have antioxidant and immune-boosting properties. This study evaluated the protective effect of dietary MLE on HS-induced liver injury and intestinal flora disturbance in Largemouth bass (Micropterus salmoides) based on oxidative damage parameters, immune parameters, and intestinal flora composition. Fish were randomly assigned into three groups: CON group (ambient temperature, 26 degrees C, basal diet), HS group (33 degrees C, basal diet), and HS + MLE group (33 degrees C, basal diet supplemented with 9 g/kg MLE). HS significantly decreased the final body weight, specific growth rate, intestinal villus length, muscular layer thickness, ACE and Chao1 indices, and lipase, trypsin, total superoxide dismutase (T-SOD), glutathione peroxidase (GPx), alkaline phosphatase (AKP), and lysozyme (LZM) activities, but significantly increased the feed conversion rate, aspartate aminotransferase (AST) activity, reactive oxygen species (ROS), malondialdehyde (MDA), complement 3 (C3) contents, and the relative abundances of Proteobacteria and Plesiomonas. Compared with the HS group, dietary MLE significantly improved lipase, trypsin, GPx, ACP, and LZM activities, and ACE and Chao1 indices in intestine, but significantly decreased the numbers of vacuoles and inflammatory cells, AST activity, and the GLU, MDA, and ROS contents. Dietary MLE also significantly up-regulated the mRNA expressions of gpx, tgf-beta, il-10, jnk2, and bcl-2, but down-regulated the mRNA expressions of il-8, tnf-alpha, caspase-3, atf4, chop, ire1, traf2, jnk1, TRPV, MCU, and VDAC in liver. In conclusion, dietary supplementation with 9 g/kg MLE could improve the growth, immunity, and diversity of intestinal flora in heat-stressed largemouth bass, and alleviate hepatic injuries by regulating inflammation, oxidative stress, and apoptosis, providing a theoretical basis for the development of MLE as a treatment against HS.
- 相关文献
作者其他论文 更多>>
-
Purification Effect and Microbial Community Analysis of Aquaculture Wastewater Using High-Efficiency and Stable Biochemical System
作者：Su, Lei;Su, Yuting;Wu, Shanshan;Gao, Hang;Wu, Hangtao;Yang, Minghui;Li, Yaying;Wang, Dan;Lu, Yusheng;Zhang, Kun;Gu, Wenjie;Peng, Huanlong;Zhou, Donglai
关键词：aquaculture wastewater; microbial community; pollutant removal; stable bioreactor
-
Targeted and non-targeted metabolic characteristics of 6,7-dihydroxy-2,4-dimethoxyphenanthrene during simulated gastrointestinal digestion
作者：Li, Qian;Liu, Fan;Liao, Sentai;Zhou, Donglai;Xing, Dongxu;Zou, Yuxiao
关键词：Phenanthrenes; Chinese yam; Gastrointestinal digestion; Characteristic metabolism
-
Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides)
作者：Zhou, Donglai;Zhong, Wenhao;Fu, Bing;Li, Erna;Li, Qingrong;Yang, Qiong;Zou, Yuxiao;Liao, Sentai;Xing, Dongxu;Zhou, Donglai;Li, Erna;Wang, Fubao;Zhong, Wenhao;Hao, Le;Liu, Zhenxing
关键词：largemouth bass; growth; serum biochemistry; liver metabolism; inflammation
-
Hsp70-mediated manipulation of antimicrobial peptide-related genes promotes BmNPV proliferation in Bombyx mori at relatively high temperatures
作者：Zhang, Jie;Hu, Yingying;Feng, Jiawei;He, Na;Li, Xiaofeng;Liyi, Qingge;Yi, Huiyu;Yang, Wanying;Zhang, Jie;Yang, Qiong
关键词：Bombyx mori; BmNPV; Innate immunity; Host-pathogen interaction; Heat shock protein (Hsp); Antimicrobial peptide (AMP)
-
Anti-Hyperuricemic and Nephroprotective Effects of Hydrolysate Derived from Silkworm Pupae (Bombyx mori): In Vitro and In Vivo Study
作者：Fan, Yuting;Yang, Zhencong;Lin, Xiao;Xu, Zhoujin;Wu, Xuli;Mu, Lixia;Li, Qingrong
关键词：silkworm pupae; peptides; anti-hyperuricemic; anti-inflammatory; xanthine oxidase
-
Lycorine provides antiviral activities against mandarin fish ranavirus via suppressing apoptosis and activating type I interferon pathway
作者：Jia, Mengyan;Guo, Hui;Jia, Mengyan;Ma, Yanping;Hao, Le;Tao, Ziqian;Wang, Jingya;Liu, Zhenxing;Tao, Ziqian;Wang, Jingya;Liao, Sentai
关键词：MRV; Lycorine; Apoptosis; Interferon; ROS
-
Comparative study of intelligent sensory technologies combined multiple metabolomics in evaluating sensory attributes and health profiles across different grades and varieties of green tea-processed mulberry leaf tea
作者：Lin, Junhui;Yang, Zhangchang;Li, Qian;Wang, Weifei;Liao, Sentai;Xing, Dongxu;Pang, Daorui;Zou, Yuxiao;Chen, Ruohong
关键词：Mulberry leaf tea; After frost; Different grades; Volatile metabolites; Nonvolatile metabolites; Bioactive substances; Lignans; Intelligent sensory technologies
