Hsp70-mediated manipulation of antimicrobial peptide-related genes promotes BmNPV proliferation in Bombyx mori at relatively high temperatures
文献类型： 外文期刊
作者： Zhang, Jie 1 ; Hu, Yingying 1 ; Feng, Jiawei 1 ; He, Na 1 ; Li, Xiaofeng 1 ; Liyi, Qingge 1 ; Yang, Qiong 3 ; Yi, Huiyu 1 ; Yang, Wanying 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Lab Lingnan Modern Agr, Lab Insect Mol Biol & Biotechnol,Guangdong Prov Ke, Guangzhou 510642, Peoples R China
2.Zunyi Med Univ, Coll Preclin Med, Dept Microbiol, Zunyi 563000, Peoples R China
3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510640, Peoples R China
关键词： Bombyx mori; BmNPV; Innate immunity; Host-pathogen interaction; Heat shock protein (Hsp); Antimicrobial peptide (AMP)
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 310 卷
页码：
收录情况： SCI
摘要： The silkworm, an economically important insect in agricultural production, are highly susceptible to various diseases caused by pathogenic microorganisms under dense group feeding conditions, particularly Bombyx mori nucleopolyhedrovirus (BmNPV), which poses a heightened infection risk at temperatures exceeding 30 degrees C, leading to severe economic losses. In this study, we investigated the environmental temperature dynamics of virus-host interactions using BmNPV. Our survival assays revealed that elevated temperatures significantly impact the proliferation and accumulation of BmNPV within the silkworm. RNA-seq and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed that Hsp70 mRNA levels were increased in response to high temperatures and BmNPV infection. Notably, while antimicrobial peptide (AMP) genes were induced by BmNPV, the expression of Hsp70 at high temperatures decreased AMP gene expression by inhibiting the nuclear translocation of nuclear transcription factor-kappa B (NF-kappa B) transcription factors. Our findings suggest that higher environmental temperatures facilitate BmNPV infection in silkworms by downregulating the expression of AMPs through the Hsp70 protein. The results of this study offer valuable insights into the interactions between BmNPV and its host under relatively high temperatures, potentially guiding strategies to mitigate viral infections in sericulture production.
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