Molecular characteristics, polymorphism and expression analysis of mhc II in yellow catfish(pelteobagrus fulvidraco)responding to Flavobacterium columnare infection
文献类型： 外文期刊
作者： Wang, Bingchao 1 ; Zhu, Fangzheng 1 ; Shi, Zechao 3 ; Huang, Zhenyu 1 ; Sun, Ruhan 1 ; Wang, Qingchao 1 ; Gang, Ouyang 2 ; Ji, Wei 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Dept Aquat Anim Med, Key Lab Freshwater Anim Breeding,Minist Agr & Rura, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
关键词： Yellow catfish (Pelteobagrus fulvidraco); mhc II; Polymorphism; Gene expression; Flavobacterium columnare
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）
ISSN： 1050-4648
年卷期： 2022 年 125 卷
页码：
收录情况： SCI
摘要： The major histocompatibility complex (MHC) is an important component of the immune system of vertebrates, which plays a vital role in presenting extrinsic antigens. In this study, we cloned and characterized the mhc IIA and mhc IIB genes of yellow catfish Pelteobagrus fulvidraco. The open reading frames (ORFs) of mhc IIA and mhc IIB genes were 708 bp and 747bp in length, encoding 235 and 248 amino acids, respectively. The structure of mhc IIA and mhc IIB includes a signal peptide, an alpha 1/beta 1 domain, an alpha 2/beta 2 domain, a transmembrane region and a cytoplasmic region. Homologous identity analysis revealed that both mhc IIA and mhc IIB shared high protein sequence similarity with that of Chinese longsnout catfish Leiocassis longirostris. mhc IIA and mhc IIB showed similar expression patterns in different tissues, with the higher expression level in spleen, head kidney and gill and lower expression in liver, stomach, gall bladder and heart. The mRNA expression level of mhc IIA and mhc IIB in different embryonic development stages also showed the similar trends. The higher expression was detected from fertilized egg to 32 cell stage, low expression from multicellular period to 3 days post hatching (dph), and then the expression increased to a higher level from 4 dph to 14 dph. The mRNA expression levels of mhc IIA and mhc IIB were significantly up-regulated not only in the body kidney and spleen, but also in the midgut, hindgut, liver and gill after challenge of Flavobacterium columnare. The results suggest that Mhc II plays an important role in the anti-infection process of yellow catfish P. fulvidraco.
- 相关文献
作者其他论文 更多>>
-
Bioinformatics characteristics and expression analysis of IL-8 and IL-10 in largemouth bass ( Micropterus salmoides ) upon Nocardia seriolae infection
作者：Wang, Qin;Zhao, Zhangchun;Sun, Ruhan;Zhang, Yaqian;Wang, Bingchao;Zhang, Xuezhen;Ji, Wei;Shi, Zechao;Ji, Wei
关键词：Largemouth bass(Micropterussalmoides); IL-8; IL-10; Inflammatory response; Nocardia seriolae
-
Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet
作者：Liu, Naicheng;Zhang, Peng;Xue, Mingyang;Zhang, Mengwei;Huang, Zhenyu;Xu, Chen;Meng, Yan;Fan, Yuding;Liu, Wei;Zhang, Feixiang;Zhou, Yong;Zhang, Feixiang;Chen, Peng
关键词：rice bran oil extract; tocotrienol (T3); zebrafish; type 2 diabetes mellitus (T2DM) model; high-fat diet; blood fat
-
Biochemical profiling of the protein encoded by grass carp reovirus genotype II
作者：Liu, Man;Kong, Xianghui;Liu, Man;Xu, Chen;Zhou, Yong;Xue, Mingyang;Jiang, Nan;Li, Yiqun;Huang, Zhenyu;Meng, Yan;Liu, Wenzhi;Fan, Yuding
关键词：
-
Bioinformatics characteristics and expression analysis of TLR3 and its adaptor protein TRIF in largemouth bass (Micropterus salmoides) upon Flavobacterium columnare infection
作者：Zhao, Zhangchun;Liu, Sixue;Wu, Chen;Wang, Qin;Zhang, Yaqian;Wang, Bingchao;Sun, Ruhan;Guo, Mengge;Ji, Wei;Wang, Long;Ji, Wei
关键词：Micropterus salmoides; TLR3; TRIF; Gene expression; Flavobacterium columnare
-
Molecular characterization and expression analyses of five genes involved in the MyD88-dependent pathway of yellow catfish (Pelteobagrus fulvidraco) responding to challenge of Aeromonas hydrophila
作者：Yuan, Yujie;Wang, Qin;Guo, Mengge;Zhao, Zhangchun;Liu, Sixue;Wu, Chen;Sun, Ruhan;Wang, Bingchao;Ji, Wei;Shi, Zechao;Yuan, Le;Ouyang, Gang;Ji, Wei
关键词：Pelteobagrus fulvidraco; MyD88; IRAK4; IRAK1; TRAF6; NF kappa B1; Gene expression; Aeromonas hydrophila
-
Molecular characterization, expression and function analysis of interleukin-17B, C and D genes in yellow catfish (Pelteobagrus fulvidraco)
作者：Zhou, Xu;Jiang, Xin-Xin;Zhang, Gui-Rong;Ji, Wei;Ma, Xu-Fa;Wei, Kai-Jian;Zhou, Xu;Jiang, Xin-Xin;Zhang, Gui-Rong;Ji, Wei;Ma, Xu-Fa;Wei, Kai-Jian;Shi, Ze-Chao
关键词：Yellow catfish; IL-17B; IL-17C; IL-17D; Expression; Bioactivity; Phagocytosis; Chemotaxis
-
Blood brain barrier permeability and immune function of brain in rainbow trout responding to IHNV infection
作者：Sun, Ru-Han;Chen, Kai-Wei;Wang, Qin;Huang, Zhen-Yu;Wang, Bing-Chao;Ji, Wei;Shi, Ze-Chao
关键词：Central nervous system; Blood-brain barrier; Immune; Infectious hematopoietic necrosis virus
