Novel insight into the antioxidant proteins derived from laver (Porphyra haitanensis) by proteomics analysis and protein based bioinformatics
文献类型： 外文期刊
作者： Pan, Chuang 1 ; Ma, Jingrong 2 ; Tao, Fengting 1 ; Ji, Chenyang 1 ; Zhao, Yongqiang 1 ; Chen, Shengjun 1 ; Yang, Xianq 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Natl R&D Ctr Aquat Prod Proc, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc,Minist Agr & Rural Affair, Guangzhou 510300, Peoples R China
2.Hainan Univ, Coll Food Sci & Engn, 58 People Rd, Haikou 570228, Hainan, Peoples R China
3.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
4.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety,K, Guangdong Prov Engn Lab Marine Biol Prod,Guangdon, Zhanjiang 524088, Peoples R China
5.Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China
关键词： Antioxidant activity; Bioactive peptides; Laver; Shotgun proteomics; Porphyra haitanensis
期刊名称：FOOD BIOSCIENCE （ 影响因子：4.24； 五年影响因子：4.805 ）
ISSN： 2212-4292
年卷期： 2021 年 42 卷
页码：
收录情况： SCI
摘要： Nowadays, new bioactive products of natural origin have been one of the main areas of research in food science and technology. In this study, a shotgun proteomics and protein based bioinformatic analysis were applied for the characterization of the marine red algae (Porphyra haitanensis) proteome. A total of 3728 proteins were identified by searching in the self-constructed P. haitanensis transcriptome repository. The predominant function of the proteins were catalytic activity, binding, transporter activity, structural molecule activity. Total of 30 proteins were identified with antioxidant activities. Network analysis revealed three main sub-works referred to alpha-linolenic acid metabolism, peroxisome, and glutathione metabolism were determined. No conserved motifs were predicted among the identified antioxidant proteins via multiple sequence alignment. The bioactive trypsin digested peptides determined by in-silico digestion have the potential to be applied as natural antioxidants for both health and food stabilization.
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