Guijing2501 (Citrus unshiu) Has Stronger Cold Tolerance Due to Higher Photoprotective Capacity as Revealed by Comparative Transcriptomic and Physiological Analysis and Overexpression of Early Light-Induced Protein
文献类型： 外文期刊
作者： Xiao, Cui 1 ; He, Ligang 1 ; Qiu, Wenming 1 ; Wang, Zeqiong 1 ; He, Xiujuan 1 ; Xiao, Yuxiong 1 ; Sun, Zhonghai 1 ; Tong, Zhu 1 ; Jiang, Yingchun 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Fruit & Tea, Hubei Key Lab Germplasm Innovat & Utilizat Fruit T, Wuhan 430064, Peoples R China
关键词： citrus; cold stress; early light-induced protein; transcriptome
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 21 期
页码：
收录情况： SCI
摘要： Cold is one of the major limiting factors for citrus production, particularly extreme cold waves. Therefore, it is of great importance to develop cold-tolerant varieties and clarify their cold tolerance mechanisms in citrus breeding. In this study, comparative transcriptomic and physiological analyses were performed to dissect the cold tolerance mechanism of Guijing2501 (GJ2501), a new satsuma mandarin (Citrus unshiu) variety with about 1 C-degrees lower LT50 (the median lethal temperature) relative to Guijing (GJ). The physiological analysis results revealed that GJ2501 is more cold-tolerant with less photoinhibition, PSII photodamage, and MDA accumulation, but higher POD activity than GJ under cold stress. Comparative transcriptomic analysis identified 4200 DEGs between GJ and GJ2501, as well as 4884 and 5580 up-regulated DEGs, and 5288 and 5862 down-regulated DEGs in response to cold stress in GJ and GJ2501, respectively. "Photosynthesis, light harvesting" and "photosystem" were the specific and most significantly enriched GO terms in GJ2501 in response to cold stress. Two CuELIP1 genes (encoding early light-induced proteins) related to the elimination of PSII photodamage and photoinhibition were remarkably up-regulated (by about 1000-fold) by cold stress in GJ2501 as indicated by RT-qPCR verification. Overexpression of CuELIP1 from GJ2501 in transgenic Arabidopsis protected PSII against photoinhibition under cold stress. Taken together, the cold tolerance of GJ2501 may be ascribed to its higher photoprotective capacity under cold stress.
- 相关文献
作者其他论文 更多>>
-
A natural mutant kumquat fruit with abnormal oil glands shows lower volatile production, better fruit flavor and higher resistance to postharvest disease
作者：Zhu, Man;Wang, Yang;Zeng, Zhebin;Wu, Xi;Teng, Jiacheng;Zhang, Jingzong;Han, Qianrong;Cheng, Yunjiang;Deng, Xiuxin;Zeng, Yunliu;Zhu, Man;Ma, Qiaoli;Chen, Chuanwu;Nieuwenhuizen, Niels J.;Atkinson, Ross G.;Xiao, Cui
关键词：Geotrichum; Limonene; Penicillium; Sensory analysis; Terpene; Transcriptomics
-
Establishment of an efficient root mediated genetic transformation method for gene function verification in citrus
作者：Xiao, Yu-Xiong;Xiao, Cui;Tong, Zhu;Wang, Ze-Qiong;He, Xiu-Juan;Sun, Zhong-Hai;Qiu, Wen-Ming;Dutt, Manjul;Ma, Hai-jie
关键词：Citrus; Genetic transformation Transgenic roots Gene; function
-
CsMYB15 positively regulates Cs4CL2-mediated lignin biosynthesis during juice sac granulation in navel orange
作者：Song, Fang;Li, Zixuan;Wang, Ce;Jiang, Yingchun;Wang, Zhijing;He, Ligang;Ma, Xiaofang;Zhang, Yu;Song, Xin;Wu, Liming;Song, Fang;Jiang, Yingchun;Liu, Jihong;Wu, Liming;Liu, Jihong
关键词：'Lane late' navel orange; granulation; transcription factor; CsMYB15; lignin biosynthesis
-
Anthocitrus: evaluation of anthocyanin accumulating "Mexican" lime fruits produced by overexpressing the Ruby transcription factor gene from Citrus sinensis "Moro"
作者：Dutt, Manjul;Mahmoud, Lamiaa M.;Weber, Kyle C.;Satpute, Aditi;Stanton, Daniel;Soares, Juliana M.;Grosser, Jude W.;Qiu, Wenming;Reuss, Laura;Wang, Yu;Killiny, Nabil
关键词：Citrus; Anthocyanins; Ruby gene; Genetic transformation; transcriptome profiling
-
A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume)
作者：Xiao, Yuxiong;Xiao, Cui;He, Xiujuan;Yang, Xin;Tong, Zhu;Wang, Zeqiong;Sun, Zhonghai;Qiu, Wenming
关键词：chestnut; CmnsLTP; CmnsLTP6.9; osmotic; drought
-
Characterization of pectin methylesterase gene family and its possible role in juice sac granulation in navel orange (Citrus sinensis Osbeck)
作者：Li, Zixuan;Wu, Liming;Wang, Ce;He, Ligang;Wang, Zhijing;Ma, Xiaofang;Bai, Fuxi;Jiang, Yingchun;Song, Fang;Li, Zixuan;Wang, Yue;Feng, Guizhi;Liu, Jihong
关键词：Citrus sinensis; Pectin methylesterases (PMEs); Juice sac granulation; Transcription factor
-
Genome-wide identification, bioinformatics characterization and functional analysis of pectin methylesterase inhibitors related to low temperature-induced juice sac granulation in navel orange (Citrus sinensis Osbeck)
作者：Li, Zixuan;Wang, Ce;Long, Di;Jiang, Yingchun;He, Ligang;Wang, Zhijing;Ma, Xiaofang;Bai, Fuxi;Wu, Liming;Song, Fang;Li, Zixuan;Liu, Jihong;Long, Di
关键词：Pectin methylesterase inhibitors (PMEIs ); Citrus; Expression pattern; Subcellular localization; Overexpression
