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Molecular characterization and transcriptional regulation analysis of the Torreya grandis squalene synthase gene involved in sitosterol biosynthesis and drought response

文献类型： 外文期刊

作者： Zhang, Feicui 1 ; Kong, Congcong 1 ; Ma, Zhenmin 1 ; Chen, Wenchao 1 ; Li, Yue 1 ; Lou, Heqiang 1 ; Wu, Jiasheng 1 ;

作者机构： 1.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Maize & Featured Dryland Crops, Dongyang, Peoples R China

关键词： Torreya grandis; transcriptional regulation; squalene synthase; sitosterol biosynthesis; drought

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： The kernel of Torreya grandis cv. 'Merrillii' (Cephalotaxaceae) is a rare nut with a variety of bioactive compounds and a high economic value. & beta;-sitosterol is not only the most abundant plant sterol but also has various biological effects, such as antimicrobial, anticancer, anti-inflammatory, lipid-lowering, antioxidant, and antidiabetic activities. In this study, a squalene synthase gene from T. grandis, TgSQS, was identified and functionally characterized. TgSQS encodes a deduced protein of 410 amino acids. Prokaryotic expression of the TgSQS protein could catalyze farnesyl diphosphate to produce squalene. Transgenic Arabidopsis plants overexpressing TgSQS showed a significant increase in the content of both squalene and & beta;-sitosterol; moreover, their drought tolerance was also stronger than that of the wild type. Transcriptome data from T. grandis seedlings showed that the expression levels of sterol biosynthesis pathway-related genes, such as HMGS, HMGR, MK, DXS, IPPI, FPPS, SQS, and DWF1, increased significantly after drought treatment. We also demonstrated that TgWRKY3 directly bound to the TgSQS promoter region and regulated its expression through a yeast one-hybrid experiment and a dual luciferase experiment. Together, these findings demonstrate that TgSQS has a positive role in & beta;-sitosterol biosynthesis and in protecting against drought stress, emphasizing its importance as a metabolic engineering tool for the simultaneous improvement of & beta;-sitosterol biosynthesis and drought tolerance.

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