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Identification of a novel bluetongue virus 1 specific B cell epitope using monoclonal antibodies against the VP2 protein

文献类型： 外文期刊

作者： Wang, Aiping 1 ; Du, Jinran 1 ; Feng, Hua 2 ; Zhou, Jingming 1 ; Chen, Yumei 1 ; Liu, Yankai 1 ; Jiang, Min 1 ; Jia, Rui; 1 ;

作者机构： 1.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

关键词： Bluetongue virus; VP2 protein; Monoclonal antibodies; Epitope

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 183 卷

页码：

收录情况： SCI

摘要： Bluetongue (BT) is a non-contact infectious disease caused by Bluetongue virus (BTV), which can be transmitted by vector insects such as Culicoides and Aedes mosquitoes. The BTV VP2 protein encoded by the L2 gene is located at the outermost layer of the virus particle, plays a key role onmediating the adsorption and entry of virus, and it is also a main antigenic protein widely used for vaccine development. In this study, the BTV1 VP2 gene was cloned into pFastBac (TM) Dual vector, and expressed in insect Sf21 cells. Immunized mice with purified recombinant VP2 protein can induce higher levels of antibodies. Three anti BTV1 VP2 monoclonal antibodies (mAbs) were generated (17E9C6, 17E9C8, 17E9H12), and showed high specific reactivity with recombinant VP2 protein and inactivated BTV1 virus. Finally, a novel linear B-cell epitope (296-)KEPAD(-300) on recombinant VP2 protein was identified by using three mAbs react with a series of continue-truncated peptides. The results of this study may provide new information on the structure and function of BTV1 VP2 protein and lay a foundation for the development of BTV1 diagnostic and prophylactic methods. (C) 2021 Elsevier B.V. All rights reserved.

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