Iridium (IV) oxide-mediated microorganism nanozyme amplified immunochromatographic assay for dual-signal sensitive detection of salbutamol
文献类型： 外文期刊
作者： Zhao, Shuang 1 ; Xu, Zhihao 1 ; Bu, Tong 1 ; Hu, Xiaofei 2 ; Cao, Jinbo 4 ; Hou, Jinjie 1 ; Bai, Feier 1 ; Zhang, Ruoxi 1 ; Wang, Li 1 ; Zhang, Gaiping 2 ;
作者机构： 1.Northwest A&F Univ, Shenzhen Res Inst, Shenzhen 518000, Peoples R China
2.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China
3.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
4.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Henan, Peoples R China
关键词： Salbutamol; Nanozyme; Immunochromatographic assay; Yeast@IrO 2; Dual-colorimetric
期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）
ISSN： 0956-7135
年卷期： 2023 年 145 卷
页码：
收录情况： SCI
摘要： Salbutamol (SAL) residues in edible animal tissues have various adverse effects on human health after digestion. Herein, we constructed an innovative functionalized microorganism nanozyme-mediated immunochromatographic assay for sensitive and accurate dual-colorimetric detection of SAL by introducing the Yeast@IrO2 as bifunctional signal tag. The Yeast@IrO2 was synthesized by self-assembling iridium (IV) oxide nanoparticles (IrO2 NPs) on the surface of inactivated yeast cells, which showed high monodispersity, uniform shapes, preeminent biocompatibility and rapid antibodies labeling capacity. After reaction for 15 min, through further employing [3,3 ',5,5' Tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2)] as substrate to trigger the color reaction, the developed biosensor performed with outstanding performance, including limit of detection (0.012 ng/mL), linearity (R2 > 0.99) and selectivity. Concurrently, the visual limit of detection (0.2 mu g/kg) and satisfactory recoveries (85.6%-103.9%) were obtained by monitoring SAL in pig liver and beef samples. Briefly, this work provided a universal platform for sensitive, precise and rapid detection of toxic and hazardous substances in food safety fields.
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