文献类型： 外文期刊

作者： Yang, Chengfeng ¹ ; Huang, Yanzhong ¹ ; Lv, Peiyun ¹ ; Antwi-Boasiako, Augustine ¹ ; Begum, Naheeda ¹ ; Zhao, Tuanjie ¹ ; Zhao, Jinming ¹ ;

作者机构： 1.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Key Lab Biol & Genet & Breeding Soybean, Minist Agr,State Key Lab Crop Genet & Germplasm E, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Anim Sci, Key Lab Saline Alkali Soil Improvement & Utilizat, Natl Forage Breeding Innovat Base JAAS,Minist Agr, Nanjing 210014, Peoples R China

3.CSIR, Crops Res Inst, AK420, Kumasi, Ghana

关键词： Glycine max; NAC transcription factors; drought tolerance; CRISPR; Cas9; protein interaction

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 19 期

页码：

收录情况： SCI

摘要： NAC transcription factors (TFs) could regulate drought stresses in plants; however, the function of NAC TFs in soybeans remains unclear. To unravel NAC TF function, we established that GmNAC12, a NAC TF from soybean (Glycine max), was involved in the manipulation of stress tolerance. The expression of GmNAC12 was significantly upregulated more than 10-fold under drought stress and more than threefold under abscisic acid (ABA) and ethylene (ETH) treatment. In order to determine the function of GmNAC12 under drought stress conditions, we generated GmNAC12 overexpression and knockout lines. The present findings showed that under drought stress, the survival rate of GmNAC12 overexpression lines increased by more than 57% compared with wild-type plants, while the survival rate of GmNAC12 knockout lines decreased by at least 46%. Furthermore, a subcellular localisation analysis showed that the GmNAC12 protein is concentrated in the nucleus of the tobacco cell. In addition, we used a yeast two-hybrid assay to identify 185 proteins that interact with GmNAC12. Gene ontology (GO) and KEGG analysis showed that GmNAC12 interaction proteins are related to chitin, chlorophyll, ubiquitin-protein transferase, and peroxidase activity. Hence, we have inferred that GmNAC12, as a key gene, could positively regulate soybean tolerance to drought stress.