CcNAC1 by Transcriptome Analysis Is Involved in Sudan Grass Secondary Cell Wall Formation as a Positive Regulator
文献类型： 外文期刊
作者： Huang, Yanzhong 1 ; Qian, Chen 1 ; Lin, Jianyu 2 ; Antwi-Boasiako, Augustine 2 ; Wu, Juanzi 1 ; Liu, Zhiwei 1 ; Mao, Zhengfeng 4 ; Zhong, Xiaoxian 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Key Lab Saline Alkali Soil Improvement & Utilizat, Minist Agr & Rural Affairs,Natl Forage Breeding In, Nanjing 210014, Peoples R China
2.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Key Lab Biol & Genet & Breeding Soybean, Minist Agr,State Key Lab Crop Genet & Germplasm En, Nanjing 210095, Peoples R China
3.CSIR, Crops Res Inst, POB 3785, Kumasi, Ghana
4.Nanjing Agr Univ, Coll Agrograssland Sci, Nanjing 210095, Peoples R China
关键词： Sudan grass; secondary cell wall; RNA-seq; CcNAC1; protein interaction
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 7 期
页码：
收录情况： SCI
摘要： Sudan grass is a high-quality forage of sorghum. The degree of lignification of Sudan grass is the main factor affecting its digestibility in ruminants such as cattle and sheep. Almost all lignocellulose in Sudan grass is stored in the secondary cell wall, but the mechanism and synthesis of the secondary cell wall in Sudan grass is still unclear. In order to study the mechanism of secondary cell wall synthesis in Sudan grass, we used an in vitro induction system of Sudan grass secondary cell wall. Through transcriptome sequencing, it was found that the NAC transcription factor CcNAC1 gene was related to the synthesis of the Sudan grass secondary cell wall. This study further generated CcNAC1 overexpression lines of Arabidopsis to study CcNAC1 gene function in secondary cell wall synthesis. It was shown that the overexpression of the CcNAC1 gene can significantly increase lignin content in Arabidopsis lines. Through subcellular localization analysis, CcNAC1 genes could be expressed in the nucleus of a plant. In addition, we used yeast two-hybrid screening to find 26 proteins interacting with CcNAC1. GO and KEGG analysis showed that CcNAC1 relates to the metabolic pathways and biosynthesis of secondary metabolites. In summary, the synthesis of secondary cell wall of Sudan grass can be regulated by CcNAC1.
- 相关文献
作者其他论文 更多>>
-
Low-cost and reliable substrate-based phenotyping platform for screening salt tolerance of cutting propagation-dependent grass, paspalum vaginatum
作者：Liu, Zhiwei;Xue, Wentao;Zhong, Xiaoxian;Liu, Zhiwei;Xue, Wentao;Jiang, Qijuan;Zhong, Xiaoxian;Liu, Zhiwei;Xue, Wentao;Zhong, Xiaoxian;Liu, Zhiwei;Olaniran, Ademola Olufolahan;Xue, Wentao;Zhong, Xiaoxian;Jiang, Qijuan
关键词：Salt tolerance; Phenotyping system; Average leaf number; Salt(50); Paspalum vaginatum
-
CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)
作者：Huang, Yanzhong;Wu, Juanzi;Liu, Zhiwei;Qian, Chen;Zhong, Xiaoxian;Lin, Jianyu;Mao, Zhengfeng
关键词：Sudan grass (Sorghum sudanense S.); secondary cell wall; bioinformatics analysis; CcNAC6; lignin
-
NAC Transcription Factor GmNAC12 Improved Drought Stress Tolerance in Soybean
作者：Yang, Chengfeng;Huang, Yanzhong;Lv, Peiyun;Antwi-Boasiako, Augustine;Begum, Naheeda;Zhao, Tuanjie;Zhao, Jinming;Huang, Yanzhong;Antwi-Boasiako, Augustine
关键词：Glycine max; NAC transcription factors; drought tolerance; CRISPR; Cas9; protein interaction
-
Improving hybrid Pennisetum growth and cadmium phytoremediation potential by using Bacillus megaterium BM18-2 spores as biofertilizer
作者：Kamal, Nehal;Liu, Zhiwei;Qian, Chen;Wu, Juanzi;Zhong, Xiaoxian;Kamal, Nehal;Liu, Zhiwei;Qian, Chen;Wu, Juanzi;Zhong, Xiaoxian;Liu, Zhiwei;Qian, Chen;Wu, Juanzi;Zhong, Xiaoxian;Kamal, Nehal
关键词：Bacterial spores; Biofertilizer; Bioremediation; BM18-2, cadmium; H. Pennisetum
-
Effect of endophytic Bacillus megaterium colonization on structure strengthening, microbial community, chemical composition and stabilization properties of Hybrid Pennisetum
作者：Shah, Assar A.;Liu, Zhiwei;Qian, Chen;Wu, Juanzi;Zhong, Xiaoxian;Shah, Assar A.;Liu, Zhiwei;Qian, Chen;Wu, Juanzi;Zhong, Xiaoxian;Shah, Assar A.;Liu, Zhiwei;Qian, Chen;Wu, Juanzi;Zhong, Xiaoxian;Umm-e-Kalsoom
关键词：Bacillus megaterium 18-2; chemical composition; fermentation quality; Lactobacillus plantarum; Hybrid pennisetum
-
Effects of natamycin and Lactobacillus plantarum on the chemical composition, microbial community, and aerobic stability of Hybrid pennisetum at different temperatures
作者：Shah, Assar Ali;Qian, Chen;Wu, Juanzi;Liu, Zhiwei;Tao, Zhujun;Zhang, Xiaomin;Zhong, Xiaoxian;Shah, Assar Ali;Qian, Chen;Wu, Juanzi;Liu, Zhiwei;Tao, Zhujun;Zhang, Xiaomin;Zhong, Xiaoxian;Shah, Assar Ali;Qian, Chen;Wu, Juanzi;Liu, Zhiwei;Tao, Zhujun;Zhang, Xiaomin;Zhong, Xiaoxian;Khan, Salman;Khan, Irfan Ullah
关键词：
-
TaNAC6s are involved in the basal and broad-spectrum resistance to powdery mildew in wheat
作者：Zhou, Weihao;Li, Ruochen;Zhou, Shuang;Zhang, Ruiqi;Xiao, Jin;Wang, Xiue;Zhang, Shouzhong;Xing, Liping;Cao, Aizhong;Qian, Chen
关键词：TaNAC6; Powdery mildew; Broad-spectrum; Basal resistance
