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Purification and characterization of a novel peroxidase from pepper ( Piper nigrum L.)

文献类型： 外文期刊

作者： Wu, Guiping 1 ; Zhang, Chaohua 1 ; Li, Xin 1 ; Niu, Zhiqiang 2 ; Jiang, Xiaojian 3 ; Zhang, Ji 3 ; You, Long 3 ; Li, Junyao 2 ; Liu, Yanan 2 ; Wu, Haifeng 4 ; Gu, Fenglin 1 ; Hu, Weicheng 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Hainan, Peoples R China

2.Yangzhou Univ, Inst Translat Med, Sch Med, Yangzhou 225009, Jiangsu, Peoples R China

3.Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China

4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev,Minist Educ, Beijing Key Lab New Drug Discovery Based Class Chi, Key Lab Bioact Subst & Resources Utilizat Chinese, Beijing 100193, Peoples R China

5.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Hainan, Peoples R China

6.Key Lab Proc Suitabil & Qual Control Special Trop, Wanning 571533, Hainan, Peoples R China

关键词： Piper nigrum; Peroxidase; Kinetic parameter; Docking study

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2024 年 214 卷

页码：

收录情况： SCI

摘要： Peroxidase (POD) isolated from Piper nigrum L. was tested against various substrates including pyrogallol, catechol, and guaiacol. It was determined that POD in its monomeric form has a molecular weight and theoretical isoelectric point calculated to be 37.43 kDa and 7.62, respectively, based on native PAGE and the amino acid sequence. Kinetic parameters, such as K m and V max , were determined for pyrogallol, catechol, and guaiacol, with pyrogallol being the most effective substrate. The enzyme exhibited peak activity at pH 5.0 to 7.0 and temperatures between 20 degrees C and 60 degrees C. However, activity significantly declined after exposure to 80 degrees C for 15 min. The presence of K+, Ba2+, citric acid, malic acid, benzoic acid, and EDTA center dot Na2 enhanced POD activity, while Zn2+, Cu2+, Fe3+, glutathione, cysteine, and ascorbic acid inhibited it. The thermal inactivation of POD at temperatures from 60 to 80 degrees C over 30 min was accurately described using the Weibull model. A threedimensional homology model of POD was constructed using SWISS-MODEL and evaluated with the SAVES system. Molecular docking was subsequently qualified using AutoDock. The binding affinities of three substrates including pyrogallol, catechol, and guaiacol were evaluated using binding free energies, and key residues identified, which acquired a deep insight into the workings of enzyme-substrate interactions.

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