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Diverse WGBS profiles of longissimus dorsi muscle in Hainan black goats and hybrid goats

文献类型： 外文期刊

作者： Ren, Yuwei 1 ; Chen, Xing 2 ; Zheng, Xinli 1 ; Wang, Feng 1 ; Sun, Ruiping 1 ; Wei, Limin 1 ; Zhang, Yan 1 ; Liu, Hailong 1 ; Lin, Yanning 1 ; Hong, Lingling 1 ; Huang, Xiaoxian 1 ; Chao, Zhe 1 ;

作者机构： 1.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Key Lab Trop Anim Breeding & Dis Res, Haikou 571100, Peoples R China

2.Wuhan Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430000, Peoples R China

关键词： Goats; Whole-genome bisulfite sequencing; DNA methylation; Muscle fiber; Vasodilatation; Glycogen storage

期刊名称：BMC GENOMIC DATA （ 影响因子：1.9； 五年影响因子：1.9 ）

ISSN：

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundGoat products have played a crucial role in meeting the dietary demands of people since the Neolithic era, giving rise to a multitude of goat breeds globally with varying characteristics and meat qualities. The primary objective of this study is to pinpoint the pivotal genes and their functions responsible for regulating muscle fiber growth in the longissimus dorsi muscle (LDM) through DNA methylation modifications in Hainan black goats and hybrid goats.MethodsWhole-genome bisulfite sequencing (WGBS) was employed to scrutinize the impact of methylation on LDM growth. This was accomplished by comparing methylation differences, gene expression, and their associations with growth-related traits.ResultsIn this study, we identified a total of 3,269 genes from differentially methylated regions (DMR), and detected 189 differentially expressed genes (DEGs) through RNA-seq analysis. Hypo DMR genes were primarily enriched in KEGG terms associated with muscle development, such as MAPK and PI3K-Akt signaling pathways. We selected 11 hub genes from the network that intersected the gene sets within DMR and DEGs, and nine genes exhibited significant correlation with one or more of the three LDM growth traits, namely area, height, and weight of loin eye muscle. Particularly, PRKG1 demonstrated a negative correlation with all three traits. The top five most crucial genes played vital roles in muscle fiber growth: FOXO3 safeguarded the myofiber's immune environment, FOXO6 was involved in myotube development and differentiation, and PRKG1 facilitated vasodilatation to release more glucose. This, in turn, accelerated the transfer of glucose from blood vessels to myofibers, regulated by ADCY5 and AKT2, ultimately ensuring glycogen storage and energy provision in muscle fibers.ConclusionThis study delved into the diverse methylation modifications affecting critical genes, which collectively contribute to the maintenance of glycogen storage around myofibers, ultimately supporting muscle fiber growth.

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