Innovations in Transgene Integration Analysis: A Comprehensive Review of Enrichment and Sequencing Strategies in Biotechnology
文献类型： 外文期刊
作者： Zhu, Zaobing 1 ; Lu, Shengtao 1 ; Wang, Hongchun 2 ; Wang, Fan 1 ; Xu, Wenting 1 ; Zhu, Yulei 2 ; Xue, Jing 2 ; Yang, Litao 1 ;
作者机构： 1.Shanghai Jiao Tong Univ, Yazhou Bay Inst Deepsea Scitech, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
3.Ningbo Water Meter Co Ltd, Ningbo 315032, Peoples R China
关键词： transgene DNA integration; targeted DNA enrichment; sequencing; CRISPR/Cas; pAgo
期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：8.2； 五年影响因子：8.5 ）
ISSN： 1944-8244
年卷期： 2025 年 17 卷 2 期
页码：
收录情况： SCI
摘要： Understanding the integration of transgene DNA (T-DNA) in transgenic crops, animals, and clinical applications is paramount for ensuring the stability and expression of inserted genes, which directly influence desired traits and therapeutic outcomes. Analyzing T-DNA integration patterns is essential for identifying potential unintended effects and evaluating the safety and environmental implications of genetically modified organisms (GMOs). This knowledge is crucial for regulatory compliance and fostering public trust in biotechnology by demonstrating transparency in genetic modifications. This review highlights recent advancements in T-DNA integration analysis, specifically focusing on targeted DNA enrichment and sequencing strategies. We examine key technologies, such as polymerase chain reaction (PCR)-based methods, hybridization capture, RNA/DNA-guided endonuclease-mediated enrichment, and high-throughput resequencing, emphasizing their contributions to enhancing precision and efficiency in transgene integration analysis. We discuss the principles, applications, and recent developments in these techniques, underscoring their critical role in advancing biotechnological products. Additionally, we address the existing challenges and future directions in the field, offering a comprehensive overview of how innovative DNA-targeted enrichment and sequencing strategies are reshaping biotechnology and genomics.
- 相关文献
作者其他论文 更多>>
-
Inactivation of Penicillium ochrochloron spores by pulsed light: Mechanism and preservation efficacy in strawberries
作者：Cui, Xiaozhen;Bassey, Anthony Pius;Wang, Fan;Xie, Shudong;Fan, Linlin;Zhu, Yongsheng;Liu, Xiaoli;Cui, Xiaozhen;Xie, Shudong;Li, Dongnan;Bin, Li
关键词：Non-thermal technology; Pulsed light; Strawberry; Fruits; Quality
-
A dual-probe one-pot RPA-CRISPR/Cas12a: a highly sensitive and rapid method for detection of Phytophthora vignae
作者：Lin, Mingze;Zhou, Zhiming;Feng, Wanzhen;Yang, Chengdong;Chen, Qinghe;Lin, Mingze;Zhou, Zhiming;Li, Zhiting;Feng, Wanzhen;Yang, Chengdong;Chen, Qinghe;Zhu, Zaobing
关键词：cowpea; dual-probes; one-pot RPA-CRISPR/Cas12a; visual detection
-
Fabrication and characterization of gelatin/carboxymethyl chitosan composite film incorporated with carvacrol and its preservation efficacy in Chinese mitten crab ( Eriocheir sinensis)
作者：Bassey, Anthony Pius;Cui, Xiaozhen;Wang, Fan;Nasiru, Mustapha Muhammad;Fan, Linlin;Liu, Xiaoli;Ibeogu, Isaiah Henry;Bako, Hadiza Kabir
关键词：Chinese mitten crab; Active packaging; Carvacrol; Bacterial diversity; Preservation; Films
-
Herbicide resistance in Leptochloa chinensis (L.) Nees populations from different regions of Jiangsu Province, China: sensitivity differences and underlying mechanisms
作者：Xu, Peng;Ju, Yawen;Fu, Yousheng;Cao, Kaige;Wang, Ke;Wang, Hongchun;Zhu, Axiu
关键词：
Leptochloa chinensis (L.) Nees; geographical populations; herbicide; cyhalofop-butyl; sensitivity; target mutation -
Field-Deployable Detection of Genetically Modified Organisms with an Integrated Method of Loop-Mediated Isothermal Amplification and CRISPR/FnCas12a
作者：Chen, Lu;Yang, Litao;Chen, Lu;Wang, Chen;Yang, Litao;Zhu, Zaobing
关键词：Cas-pfLAMP; DBN9936; MON810; GTS 40-3-2; on-site detection; LAMP; CRISPR/Cas12a; lateral flow strip
-
HIV-1 latency: From acquaintance to confidant
作者：Yang, Chenbo;Xue, Jing;Yang, Chenbo;Xue, Jing;Xue, Jing;Tong, Ling
关键词：HIV-1 latency; Reservoir; Viral persistence; CRISPR screening; Viral eradication
-
Green synthesis of silver nanoparticles using Houttuynia cordata Thunb rhizome extracts and their antibacterial potential against common foodborne pathogens
作者：Maimaiti, Xiayidan;Liu, Xiaoli;Rahman, Nurgul;Luo, Runmeng;Maimaiti, Xiayidan;Bassey, Anthony Pius;Liu, Xiaoli;Zhu, Yongsheng;Fan, Linlin;Luo, Runmeng;Wang, Fan;Wang, Ying
关键词：Antibacterial activity; green synthesis; Houttuynia cordata; particle size; silver nanoparticles
