Development of novel specific molecular markers for the Sw-5b gene to assist with tomato spotted wilt virus-resistant tomato breeding
文献类型： 外文期刊
作者： Tong, Cong 1 ; Huang, Shen 1 ; Shi, Yajie 1 ; Wu, Qian 1 ; Shangguan, Lingna 1 ; Yu, Haohua 1 ; Chen, Rongzhen 1 ; Ding, Zixuan 1 ; Xiao, Yunxia 1 ; Ji, Yinghua 2 ; Zhang, Zhongkai 3 ; Zhang, Hui 4 ; Zhu, Min 1 ; Tao, Xiaorong 1 ;
作者机构： 1.Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Natl Tobacco Genet Engn Res Ctr, Key Lab Tobacco Biotechnol Breeding, Kunming 650021, Yunnan, Peoples R China
4.Shanghai Acad Agr Sci, Inst Hort Sci, Shanghai 201403, Peoples R China
关键词： Sw-5b; TSWV; Gene-specific molecular marker; Marker-assisted selection; Breeding; Tomato
期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.4； 五年影响因子：4.2 ）
ISSN： 2096-5362
年卷期： 2023 年 5 卷 1 期
页码：
收录情况： SCI
摘要： Tomato spotted wilt virus (TSWV) is a plant pathogen that causes devastating tomato yield losses worldwide. The Sw-5b gene is one of the most effective resistance genes for TSWV control in tomato plants, and has been widely used in resistance breeding. Molecular markers are specific DNA sequences with known locations on the chromosome; they are indispensable tools in marker-assisted selection, which detects the presence of target genes to expedite breeding. We developed gene-specific molecular markers for Sw-5b to facilitate the accurate distinction of resistance (Sw-5b(R)) and susceptibility (Sw-5b(S)) alleles of Sw-5b. Using these markers, we successfully detected Sw-5b and determined its genotype (homozygous Sw-5b(R), heterozygous Sw-5b(R/S), or homozygous Sw-5b(S)) in six tomato varieties. Then we successfully applied these markers to 46 commercial tomato cultivars to detect and determine the genotype of Sw-5b. The results revealed a striking absence of the Sw-5b(R) gene and high TSWV susceptibility among most of the analyzed commercial cultivars. With the assistance of the novel Sw-5b-specific molecular markers, we generated a TSWV-resistant and homozygous Sw-5b(R) Micro-Tom tomato line, demonstrating the practical application of these markers in plant breeding. In summary, we developed novel gene-specific molecular markers for Sw-5b, and applied them to distinguish Sw-5b alleles for TSWV resistance or susceptibility. This marker set provides a valuable tool for breeding TSWV-resistant tomato varieties.
- 相关文献
作者其他论文 更多>>
-
Self-assembly of tamarind seed polysaccharide via enzymatic depolymerization and degalactosylation enhanced ice recrystallization inhibition activity
作者：Sun, Xianbao;Guo, Rui;Kou, Yuxing;Ma, Xuan;Song, Hong;Song, Lihua;Li, Xujiao;Wu, Yan;Zhan, Taijie;Zhang, Hui;Xie, Fan;Li, Xujiao;Song, Zibo;Yuan, Chunmei
关键词：Polysaccharide; Enzymatic modification; Self-assembly; Ice recrystallization inhibition; Cryoprotection; Molecular dynamics simulation
-
Hydrophobic aggregation via partial Gal removal affects solution characteristics and fine structure of tamarind kernel polysaccharides
作者：Guo, Rui;Sun, Xianbao;Kou, Yuxing;Song, Hong;Li, Xujiao;Song, Lihua;Wu, Yan;Zhao, Taolei;Zhang, Hui;Li, Deshun;Liu, Yanfang;Song, Zibo;Li, Xujiao;Wu, Jieli;Zhao, Taolei
关键词：Tamarindus indica L; Tamarind kernel polysaccharide; Hydrophobic aggregation; Partial degalactosylation; Solution characteristics; Structural characteristics
-
NLRs derepress MED10b-and MED7-mediated repression of jasmonate- dependent transcription to activate immunity
作者：Wu, Qian;Tong, Cong;Chen, Zhengqiang;Huang, Shen;Hong, Hao;Li, Jia;Feng, Mingfeng;Wang, Huiyuan;Xu, Min;Yan, Yuling;Cui, Hongmin;Shen, Danyu;Ai, Gan;Xu, Yi;Dong, Suomeng;Wang, Xuan;Zhu, Min;Tao, Xiaorong;Zhao, Xiaohui;Wang, Huiyuan;Li, Junming;Zhang, Hui;Huang, Changjun;Zhang, Zhongkai;Dinesh-Kumar, Savithramma P.;Dinesh-Kumar, Savithramma P.
关键词：Mediator complex; NLRs; Sw-5b; effector-triggered immunity
-
A conserved clathrin-coated vesicle component, OsSCYL2, regulates plant innate immunity in rice
作者：Yao, Yao;Zhou, Jihua;Cheng, Can;Niu, Fuan;Zhang, Anpeng;Sun, Bin;Tu, Rongjian;Li, Yao;Huang, Yiwen;Xie, Kaizhen;Dai, Yuting;Cao, Liming;Chu, Huangwei;Yao, Yao;Huang, Yiwen;Dai, Yuting;Pan, Xiaohua;Wan, Jianing;Li, Yao;Xie, Kaizhen;Zhang, Hui;Hong, Jing Han;Zhu, Jiaojiao;Zhou, Hong;Liu, Zhenhua
关键词：cell death; clathrin-mediated vesicle trafficking; disease resistance; lesion mimic mutant; Oryza sativa
-
Molecular aggregation via partial Gal removal affects physicochemical and macromolecular properties of tamarind kernel polysaccharides
作者：Guo, Rui;Li, Xujiao;Sun, Xianbao;Kou, Yuxing;Zhang, Jun'ai;Wu, Yan;Li, Deshun;Liu, Yanfang;Zhao, Taolei;Zhang, Hui;Li, Xujiao;Song, Zibo;Song, Zibo
关键词：Tamarindus indica L.; Tamarind kernel polysaccharide; Molecular aggregation; Partial degalactosylation; Physicochemical properties; Macromolecular properties
-
Pectic polysaccharides from Biluochun Tea: A comparative study in macromolecular characteristics, fine structures and radical scavenging activities in vitro
作者：Guo, Rui;Zhang, Jun'ai;Liu, Xin;Li, Xujiao;Sun, Xianbao;Kou, Yuxing;Wu, Yan;Li, Deshun;Liu, Yanfang;Zhang, Hui
关键词：Camellia sinensis L.; Biluochun Tea; Pectic polysaccharides; Macromolecular characteristics; Structural elucidations; Radical scavenging activities in vitro
-
Metabolomic Analysis, Combined with Enzymatic and Transcriptome Assays, to Reveal the Browning Resistance Mechanism of Fresh-Cut Eggplant
作者：Liu, Xiaohui;Xiao, Kai;Zhang, Aidong;Zhu, Weimin;Zhang, Hui;Tan, Feng;Huang, Qianru;Wu, Xuexia;Zha, Dingshi;Liu, Xiaohui
关键词：Solanum melongena; browning; transcriptome; metabolome; membrane lipid metabolism
