Genome-Wide Identification and Characterization of Universal Stress Protein (USP) Family Members in Lycium barbarum and Transcriptional Pattern Analysis in Response to Salt Stress
文献类型： 外文期刊
作者： Lu, Jintao 1 ; Bai, Mengyao 1 ; Zhao, Jianhua 3 ; Meng, Dong 4 ; Lin, Shanzhi 1 ; Xiu, Yu 1 ; Chen, Yuchao 2 ;
作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol,Tree & Ornamental Plant, Natl Engn Lab Tree Breeding,Natl Forestry & Grassl, Minist Educ,Key Lab Genet & Breeding Forest Trees, Beijing 100083, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Agr Biotechnol Res Ctr, Yinchuan 750002, Peoples R China
3.North Minzu Univ, Coll Biol Sci & Engn, Yinchuan 750021, Peoples R China
4.Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China
关键词：
universal stress proteins;
期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 11 卷 8 期
页码：
收录情况： SCI
摘要： Lycium barbarum is a traditional medicinal and edible plant species in China, exhibiting notable salt tolerance that enables cultivation in salt-affected soils. However, intensifying soil salinization has rendered severe salt stress a critical limiting factor for its fruit yield and quality. Universal stress proteins (USPs) serve as crucial regulators for plant abiotic stress responses through developmental process modulation. Nevertheless, the characteristics and functional divergence of USP gene family members remain unexplored in L. barbarum. Here, we performed genome-wide identification and characterization of the USP gene family in L. barbarum, revealing 52 members unevenly distributed across all 12 chromosomes. Phylogenetic analysis classified these LbUSP members into four distinct groups, demonstrating the integration of the conserved USP domain and diverse motifs within each group. Collinearity analysis indicated a stronger synteny of LbUSPs with orthologs in Solanum lycopersicum than with other species (Arabidopsis thaliana, Vitis vinifera, and Oryza sativa), demonstrating that gene duplication coupled with functional conservation represented the primary mechanism underlying USP family expansion in L. barbarum. In silico promoter screening detected abundant cis-acting elements associated with abiotic/biotic stress responses (MYB and MYC binding sites), phytohormone regulation (ABRE motif), and growth/development processes (Box-4 and G-box). Transcriptome sequencing and RT-qPCR validation revealed tissue-specific differential expression patterns of LbUSP8, LbUSP11, LbUSP12, LbUSP23, and LbUSP25 in roots and stems under salt stress, identifying them as prime candidates for mediating salt resistance in L. barbarum. Our findings establish a foundation for the functional characterization of LbUSPs and molecular breeding of salt-tolerant L. barbarum cultivars.
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