Development of EST-SSR markers for an endangered plant species, Camellia fascicularis (Theaceae)
文献类型： 外文期刊
作者： Ma, Luyao 1 ; Liu, Cheng 1 ; Xin, Jing 2 ; Li, Bin 3 ; Tang, Junrong 2 ; Xin, Yaxuan 2 ; Ye, Peng 1 ; Xin, Peiyao 1 ; Bai, Bin 4 ;
作者机构： 1.Southwest Forestry Univ, Southwest Res Ctr Landscape Architecture Engn, State Forestry & Grassland Adm, Kunming 650224, Peoples R China
2.Southwest Forestry Univ, Key Lab Biodivers Conservat Southwest Reg State Fo, Kunming 650224, Yunnan, Peoples R China
3.Bijie Reg Forestry Sci Res Inst, Bijie 551700, Peoples R China
4.Gansu Acad Agr Sci, Wheat Res Inst, Lanzhou 730070, Peoples R China
关键词： Camellia fascicularis; EST-SSR; genetic diversity; Theaceae; transcriptome sequencing
期刊名称：PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION （ 影响因子：1.1； 五年影响因子：1.2 ）
ISSN： 1479-2621
年卷期： 2023 年
页码：
收录情况： SCI
摘要： The plant Camellia fascicularis, belonging to family Theaceae, has high ornamental and medicinal value, and rare gene resources for genetic improvement of Camellia crops, but is currently threatened with extinction because of the unique and extremely small wild populations. Molecular markers have clarified the wild plant species' genetic diversity structure, new gene resources and relationship with crops. This will be beneficial for conservation of these valuable crop-related wild species and crop improvement. In this study, we identified 95,979 microsatellite loci from 155,011 transcriptome unigenes, and developed 14 polymorphic expressed sequence tag-derived simple sequence repeat (EST-SSR) microsatellite markers for C. fascicularis. The number of alleles (Na) per locus was 2-8 with a mean of 4.86. The genetic diversity of 40 individuals from four natural populations of C. fascicularis was analysed using these polymorphic markers. The number of alleles (Na) for EST-SSR ranged from 2 to 5, with the expected heterozygosities (He) and observed heterozygosities (Ho) in all loci ranging from 0.183 to 0.683, and from 0.201 to 0.700, respectively, implying a rich genetic variation present in wild C. fascicularis populations. Moreover, the phylogenetic analysis among four populations, using the 14 EST-SSR markers developed in this study, grouped 40 individuals into three groups, which coincide with their geographic distribution. These results showed that 14 EST-SSR markers are available for the analysis of genetic variation in C. fascicularis populations and genetic improvement of new Camellias cultivars by interspecific hybridization, and are beneficial for conservation of the endangered species.
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