Niacin Improves Intestinal Health through Up-Regulation of AQPs Expression Induced by GPR109A
文献类型： 外文期刊
作者： Liu, Shilong 1 ; Qiu, Yueqin 1 ; Gu, Fang 1 ; Xu, Xiaoming 1 ; Wu, Shansen 1 ; Jin, Zhenhao 1 ; Wang, Li 1 ; Gao, Kaiguo 1 ; Zhu, Cui 1 ; Yang, Xuefen 1 ; Jiang, Zongyong 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci,Guangdong Lab Lingnan Modern Agr, Maoming Branch,Minist Agr & Rural Affairs,Guangdo, State Key Lab Livestock & Poultry Breeding,Key La, Guangzhou 510640, Peoples R China
关键词： piglets; IPEC-J2 cell; niacin; aquaporins; diarrhea; GPR109A
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 15 期
页码：
收录情况： SCI
摘要： (1) Background: Changes in the expression of aquaporins (AQPs) in the intestine are proved to be associated with the attenuation of diarrhea. Diarrhea is a severe problem for postweaning piglets. Therefore, this study aimed to investigate whether niacin could alleviate diarrhea in weaned piglets by regulating AQPs expression and the underlying mechanisms; (2) Methods: 72 weaned piglets (Duroc x (Landrace x Yorkshire), 21 d old, 6.60 +/- 0.05 kg) were randomly allotted into 3 groups for a 14-day feeding trial. Each treatment group included 6 replicate pens and each pen included 4 barrows (n = 24/treatment). Piglets were fed a basal diet (CON), a basal diet supplemented with 20.4 mg niacin/kg diet (NA) or the basal diet administered an antagonist for the GPR109A receptor (MPN). Additionally, an established porcine intestinal epithelial cell line (IPEC-J2) was used to investigate the protective effects and underlying mechanism of niacin on AQPs expression after Escherichia coli K88 (ETEC K88) treatment; (3) Results: Piglets fed niacin-supplemented diet had significantly decreased diarrhea rate, and increased mRNA and protein level of ZO-1, AQP 1 and AQP 3 in the colon compared with those administered a fed diet supplemented with an antagonist (p < 0.05). In addition, ETEC K88 treatment significantly reduced the cell viability, cell migration, and mRNA and protein expression of AQP1, AQP3, AQP7, AQP9, AQP11, and GPR109A in IPEC-J2 cells (p < 0.05). However, supplementation with niacin significantly prevented the ETEC K88-induced decline in the cell viability, cell migration, and the expression level of AQPs mRNA and protein in IPEC-J2 cells (p < 0.05). Furthermore, siRNA GPR109A knockdown significantly abrogated the protective effect of niacin on ETEC K88-induced cell damage (p < 0.05); (4) Conclusions: Niacin supplementation increased AQPs and ZO-1 expression to reduce diarrhea and intestinal damage through GPR109A pathway in weaned piglets.
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