Molecular networking and equivalently quantitative ion strategy for discovery and quantification of glucosinolates in cauliflower and broccoli by liquid chromatography tandem mass spectrometry
文献类型： 外文期刊
作者： Hengchao, E. 1 ; Peng, Shuting 1 ; Zhao, Zhiyong 1 ; Yao, Xueqin 2 ; Zhang, Yanmei 1 ; Li, Xiaobei 1 ; Yang, Xianli 1 ; Fan, Tingting 1 ; Zhao, Xiaoyan 1 ; Zhou, Changyan 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
2.Shanghai Acad Agr Sci, Hort Res Inst, 1018 Jinqi Rd, Shanghai 201403, Peoples R China
关键词： Glucosinolates; Molecular networking; Equivalently quantitative ion method; Cauliflower and broccoli; LC-MS/MS
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）
ISSN： 0023-6438
年卷期： 2023 年 187 卷
页码：
收录情况： SCI
摘要： Glucosinolates (GSLs) are unique secondary metabolites in cauliflower and broccoli, which have diverse biological activities. In this study, a molecular networking combined with the equivalently quantitative ion strategy was built based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the discovery and quantification of GSLs in cauliflower and broccoli. Firstly, 19 GSLs were identified including 2 new compounds, 4-hydroxyneoglucobrassicin and gluco-4-hydroxyneoglucobrassicin. Moreover, the shared fragment ion m/z 96.0 was set as the equivalently quantitative ion to quantify GSLs. This method could use only one reference standard (or one calibration curve) to efficiently quantify all aliphatic and phenyl GSLs in cauliflower and broccoli. This method was fully validated with good linearity, recovery and sensitivity, and subsequently applied in GSLs quantification. The developed strategy could also be applied in GSLs discovery and quantification in other Brassicaceae vegetables.
- 相关文献
作者其他论文 更多>>
-
Boosted antibiotic elimination over 2D/2D mesoporous CeO2/BiOCl S-scheme photocatalyst
作者：Tang, Huiling;Wang, Xuesheng;Zhang, Zhijie;Hengchao, E.;Zhou, Jiaxin;Song, Wei
关键词：S-scheme heterojunction; CeO2; BiOCl; Photocatalyst; Antibiotics
-
Risk assessment of Bacillus cereus in lettuce grown in open-field and greenhouse farming systems in China: A comparative study
作者：Qu, Yang;Zhou, Changyan;Suo, Yujuan;Yu, Shenghao;Gu, Haotian;Wang, Wen;Wang, Wenbo;He, Zhaoying;Shan, Hong;Gao, Min;Zhao, Xin;Wei, Chao;Tao, Tingting;Zhao, Guanghua
关键词：Diarrheal syndrome food poisoning; ready-to-eat vegetable; greenhouse; microbial risk assessment
-
Modeling Growth Kinetics of Escherichia coli and Background Microflora in Hydroponically Grown Lettuce
作者：You, Xiaoyan;Yang, Dongqun;Yang, Dongqun;Qu, Yang;Zhao, Xiaoyan;Suo, Yujuan;Guo, Mingming;Zhang, Yangping
关键词：growth model; hydroponically grown lettuce; Escherichia coli; background microflora
-
Formaldehyde levels and formaldehyde-exposure-related health risk levels of edible mushrooms cultivated in China
作者：Shao, Yi;Liu, Haiyan;Huang, Liujuan;Zhao, Xiaoyan;Zhao, Zhiyong;Yang, Xianli;Shao, Yi;Liu, Haiyan;Zhao, Xiaoyan;Zhao, Zhiyong;Yang, Xianli;Zhou, Changyan;Zhou, Changyan
关键词：Edible mushrooms; Formaldehyde prevalence; Naturally produced formaldehyde; Dietary risk assessment; Point assessment; Probability assessment; Chinese inhabitants
-
Detection of gyromitrin-induced mushroom poisoning by an isotope-coded derivatization strategy combined with UPLC-MS/MS and its application
作者：Fan, Tingting;He, Xiangwei;Hengchao, E.;Zhang, Yanmei;Li, Xiaobei;Li, Xujiao;Yang, Xianli;Zhou, Changyan;Zhao, Zhiyong;Zhao, Xiaoyan;Zhao, Zhiyong
关键词：Biomarker; Detection; Gyromitrin; Isotope-coded Derivatization; UPLC-MS/MS
-
Screening and dietary exposure assessment of T-2 toxin and its modified forms in commercial cereals and cereal-based products in Shanghai
作者：Guo, Wenbo;Feng, Disen;Yang, Xianli;Zhao, Zhihui;Yang, Junhua;Guo, Wenbo
关键词：T-2 toxin; Modified mycotoxins; Trichothecenes; UPLC-MS/MS; Cereals and cereal-based products; Dietary exposure assessment
-
Phylogenetic Variation of Tri1 Gene and Development of PCR-RFLP Analysis for the Identification of NX Genotypes in Fusarium graminearum Species Complex
作者：Gao, Meiling;Zhang, Mengyuan;Zhang, Jiahui;Yang, Xianli;Wang, Jianhua;Gao, Meiling;Abdallah, Mohamed F.;Abdallah, Mohamed F.
关键词：Fusarium graminearum species complex; trichothecenes; PCR-RFLP; NX toxins
