Generation and evaluation of Salmonella entericaserovar Choleraesuis mutant strains as a potential live-attenuated vaccine
文献类型： 外文期刊
作者： Huang, Xin 1 ; Yang, Shanshan 1 ; Zhao, Jing 1 ; Yang, Jing 1 ; Jiang, Huazheng 1 ; Li, Shuxian 1 ; Wang, Caiying 1 ; Liu, Guangliang 1 ;
作者机构： 1.Lanzhou Univ, State Key Lab Anim Dis Control & Prevent, Coll Vet Med, Lanzhou Vet Res Inst,Chinese Acad Agr Sci, Lanzhou, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Jiangsu, Peoples R China
3.Univ Liege, Mol & Cellular Epigenet GIGA & Mol Biol TERRA, Liege, Belgium
4.Xinjiang Agr Univ, Coll Vet Med, Urumqi, Peoples R China
5.Wageningen Univ & Res, Cell Biol & Immunol Grp, Wageningen, Netherlands
关键词： Salmonella enterica serovar Choleraesuis; CRISPR-Cas9; rpoS; slyA; Mucosal immunity
期刊名称：VACCINE （ 影响因子：3.5； 五年影响因子：3.5 ）
ISSN： 0264-410X
年卷期： 2024 年 42 卷 24 期
页码：
收录情况： SCI
摘要： Background: Salmonella entericaserovar Choleraesuis (S. C) is a swine enteric pathogen causing paratyphoid fever, enterocolitis, and septicemia in piglets. S. C is mainly transmitted through the fecal-oral route. Vaccination is an effective strategy for preventing and controlling Salmonella infection. Results: Herein, we used CRISPR-Cas9 technology to knockout the virulence regulatory genes, rpoS, and slyA of S. C and constructed the drpoS, dslyA, and drpoS dslyA strains. The phenotypic characteristics of the mutant strains remained unchanged compared with the parental wild-type strain. In vivo study, unlike the wild-type strain, the dslyA and drpoS dslyA strains alleviated splenomegaly, colon atrophy, and lower bacterial loads in the spleen, liver, ileum, and colon. These mutant strains survived in Peyer's patches (PPs) and mesenteric lymph nodes (MLN) for up to 15 days post-infection. Furthermore, the immunization of the drpoS dslyA strain induced robust humoral and cellular immune responses. Conclusions: Consequently, vaccination with drpoS dslyA conferred a high percentage of protection against lethal invasive Salmonella, specifically S. C, in mice. This study provided novel insights into the development of live-attenuated vaccines against the infection of S. C.
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