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Non-coding RNA regulates phage sensitivity in Listeria monocytogenes

文献类型： 外文期刊

作者： Tian, Yuan 1 ; Wu, Liting 2 ; Zhu, Mengya 2 ; Yang, Zhenquan 3 ; Pilar, Garcia 4 ; Bao, Hongduo 2 ; Zhou, Yan 2 ; Wang, Ran 2 ; Zhang, Hui 2 ;

作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base MOST, Nanjing, Peoples R China

3.Yangzhou Univ, Coll Food Sci & Engn, Yangzhou, Jiangsu, Peoples R China

4.Inst Prod Lacteos Asturias IPLA CSIC, Asturias, Spain

期刊名称：PLOS ONE （ 影响因子：3.752； 五年影响因子：4.069 ）

ISSN： 1932-6203

年卷期： 2021 年 16 卷 12 期

页码：

收录情况： SCI

摘要： Non-coding RNAs (ncRNAs) have gained increasing attention as their diverse roles in virulence and environmental stress in Listeria monocytogenes have become clearer. The ncRNA rliB is an atypical member of the CRISPR family, conserved at the same genomic locus in all analyzed L. monocytogenes genomes and also in other Listeria species. In this study, rliB defective mutants (Lm3-22-Delta rliB) were constructed by homologous recombination. The growth cycle of Lm3-22-Delta rliB mutants was slower than that of wild-type Lm3-22. The sensitivity of Lm3-22-Delta rliB to the Listeria phage vB-LmoM-SH3-3 was significantly increased, and the efficiency of plaque formation was enhanced by 128 fold. Compared with wild type, the adhesion and invasion of Lm3-22-Delta rliB decreased significantly (9.3% and 1.33%, respectively). After 4 hours of infection, the proliferation of Lm3-22-Delta rliB in RAW264.7 cells also decreased significantly. Transcription level of invasion-related surface proteins showed that the internalin genes lmo0610 and lm0514, and the peptidoglycan binding protein gene lmo1799 in Lm3-22-Delta rliB were significantly increased. In addition, after interaction with phage, the transcription levels of inlA, lmo0610, lmo1799, lmo2085, and lmo0514 in Lm3-22-Delta rliB cells were significantly upregulated, while inlB was downregulated, compared with Lm3-22 control group with phage treatment. Therefore, rliB deletion effectively regulated the interaction between Listeria and phage, weaken its invasion ability, and provided a new theoretical basis for biocontrol of phage.

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