Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles
文献类型： 外文期刊
作者： Li, Jian-Qiao 1 ; Zhu, Rui 1 ; Yao, Wei-Chen 1 ; Yu, Hui-Ping 1 ; Huang, Jian-Rong 2 ; Wang, Zhen 1 ; Sun, Xin-Yue 1 ; Yuan, Di-Hua 1 ; Sun, Yuan-Yuan 1 ; Emam, Sekina S. 3 ; Dewer, Youssef 3 ; Zhu, Xiu-Yun 1 ; Zhang, Ya-Nan 1 ;
作者机构： 1.Huaibei Normal Univ, Coll Life Sci, Huaibei 235000, Peoples R China
2.Henan Acad Agr Sci, Henan Key Lab Crop Pest Control, MOAs Reg Key Lab Crop IPM Southern Part Northern C, Inst Plant Protect, Zhengzhou 450002, Peoples R China
3.Agr Res Ctr, Phytotox Res Dept, Cent Agr Pesticide Lab, Giza 12618, Egypt
关键词： Athetis lepigone; chemosensory protein; sex pheromone; maize volatile; site-directed mutagenesis; RNAi
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 16 期
页码：
收录情况： SCI
摘要： In moths, the interactions between chemosensory proteins (CSPs) and sex pheromones have yet to be comprehensively investigated. Here, we examined the function of AlepCSP2 in male Athetis lepigone based on protein expression, molecular docking, site-directed mutagenesis, fluorescence competitive binding analyses, and RNA interference (RNAi) experiments. We found that AlepCSP2 showed strong binding affinity for two sex pheromones and five maize volatiles and that binding was optimal under neutral conditions. Furthermore, we identified six amino acids as being key residues involved in the interaction between AlepCSP2 and multiple ligands. Further RNAi showed that siCSP2 males displayed consistently lower electro-antennography responses to two sex pheromones and three maize volatiles at different dosages tested, and the mating rate also decreased significantly by 37.50%. These findings will contribute to characterizing the binding mechanisms of moth CSPs to sex pheromones and host volatiles and also identify unique targets for developing novel pest behavior disruptors.
- 相关文献
作者其他论文 更多>>
-
Molecule characterization of chemosensory and metabolism-related genes in the proboscis of Athetis lepigone
作者：Tian, Cai-Hong;Xu, Cun-Yi;Huang, Jian-Rong;Wang, Gen-Song;Zhang, Jun-Yi;Li, Guo-Ping;Feng, Hong-Qiang;Liu, Xiao-Guang;Zhang, Jun-Yi;Yin, Xin-Ming;Fu, Jun-Feng
关键词：Athetis lepigone; scanning electron microscopy; proboscis transcriptome; chemosensory genes; metabolism-related genes; quantitative real-time PCR
-
Effects of X-Ray Irradiation on Biological Parameters and Induced Sterility of Ephestia elutella: Establishing the Optimum Irradiation Dose and Stage
作者：Zhao, Jun;Li, Shujun;Li, Chengjun;Xu, Lu;Li, Qi;Dewer, Youssef;Wu, Kongming
关键词：Ephestia elutella; sterile insect technique; X-ray irradiation; induced sterility; male mating competitiveness
-
Highly sensitive and selective detection of butachlor based on the resonance light scattering of doped carbon quantum dots
作者：Lei, Han;Liu, Qinghao;Wang, Cundong;Xu, Mingyue;An, Wenqing;Bao, Chenning;Wang, Zhen;Leng, Jiapeng;Liu, Hongyan
关键词：
-
Application of transcriptomic analysis to unveil the toxicity mechanisms of fall armyworm response after exposure to sublethal chlorantraniliprole
作者：Xu, Lu;Xu, Dejin;Xu, Guangchun;Gu, Zhongyan;Zhao, Jun;Xiao, Zheng;Dewer, Youssef;Zhang, Yanan
关键词：Spodoptera frugiperda; Chlorantraniliprole; Toxicity mechanism; Transcriptome analysis; Sublethal concentration; Enzyme activity
-
AlepPBP2, but not AlepPBP3, may involve in the recognition of sex pheromones and maize volatiles in Athetis lepigone
作者：Yang, Hui-Hui;Xu, Ji-Wei;Li, Lu-Lu;Yao, Wei-Chen;Zhao, Pan-Pan;Zhang, Dong;Liu, Jia-Yi;Zhu, Xiu-Yun;Li, Xiao-Ming;Zhang, Ya-Nan;Zhang, Xiao-Qing;Huang, Jian-Rong;Liu, Jia-Yi;Dewer, Youssef
关键词：Athetis lepigone; fluorescence competitive binding assays; host plant volatiles; pheromone-binding proteins; sex pheromones
-
The green synthesis of carbon quantum dots and applications for sulcotrione detection and anti-pathogen activities
作者：Wang, Zhen;Liu, Qinghao;Zhang, Yuxin;Wang, Cundong;An, Wenqing;Bao, Chenning;Lei, Han;Leng, Jiapeng;Liu, Hongyan
关键词：Carbon quantum dots; Moringa oleifera roots; Sulcotrione; Inhibitory rates
-
A NEW NORTHERN DISTRIBUTION RECORD OF THE PURPLE STEM BORER, SESAMIA INFERENS (WALKER) (LEPIDOPTERA: NOCTUIDAE) IN CHINA
作者：Zhang, Yuan-chen;Feng, Hong-qiang;Huang, Jian-rong
关键词：
