文献类型： 外文期刊

作者： Du, Xue ¹ ; Xu, Xiaoqin ³ ; Liu, Yali ⁴ ; Wang, Zhijun ¹ ; Qiu, Hao ¹ ; Zhao, Ayong ¹ ; Lu, Lizhi ⁵ ;

作者机构： 1.Zhejiang A&F Univ, Coll Anim Sci & Technol,Zhejiang Int Sci & Techno, Key Lab Appl Technol Green Ecohlth Anim Husb Zhej, Zhejiang Prov Engn Lab Anim Hlth Inspect & Intern, Hangzhou 311300, Peoples R China

2.Zhejiang A&F Univ, Coll Vet Med, Hangzhou 311300, Peoples R China

3.China West Normal Univ, Inst Ecol, Nanchong 637002, Peoples R China

4.Zhejiang Prov Anim Husb Technol Promot & Breeding, Hangzhou 310020, Peoples R China

5.Zhejiang Acad Agr Sci, Inst Anim Sci & Vet,Minist Agr & Rural Affairs Ch, Key Lab Livestock & Poultry Resources Poultry Eva, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

关键词： ceased-laying duck; oviduct regression; THY1; TIMP4; cell heterogeneity

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Simple Summary This study investigated the molecular mechanisms of oviduct regression in ducks. Single-cell transcriptome sequencing of magnum tissue from egg-laying and ceased-laying ducks revealed significant heterogeneity, particularly in protein secretion cells and ECM-producing fibroblasts. Egg-laying ducks exhibited more protein secretion cells, crucial for albumen deposition, and higher proportions of THY1+ and TIMP4+ fibroblasts compared to ceased-laying ducks. These findings imply a correlation between THY1 and TIMP4 expression in fibroblasts and oviduct activity during reproduction. The study provides valuable insights into reproductive tract degeneration and potential improvements in laying duck production efficiency.Abstract Duck egg production, like that of laying hens, follows a typical low-peak-low cycle, reflecting the dynamics of the reproductive system. Post-peak, some ducks undergo a cessation of egg laying, indicative of a regression process in the oviduct. Notably, the magnum, being the longest segment of the oviduct, plays a crucial role in protein secretion. Despite its significance, few studies have investigated the molecular mechanisms underlying oviduct regression in ducks that have ceased laying eggs. In this study, we conducted single-cell transcriptome sequencing on the magnum tissue of Shaoxing ducks at 467 days of age, utilizing the 10x Genomics platform. This approach allowed us to generate a detailed magnum transcriptome map of both egg-laying and ceased-laying ducks. We collected transcriptome data from 13,708 individual cells, which were then subjected to computational analysis, resulting in the identification of 27 distinct cell clusters. Marker genes were subsequently employed to categorize these clusters into specific cell types. Our analysis revealed notable heterogeneity in magnum cells between the egg-laying and ceased-laying ducks, primarily characterized by variations in cells involved in protein secretion and extracellular matrix (ECM)-producing fibroblasts. Specifically, cells engaged in protein secretion were predominantly observed in the egg-laying ducks, indicative of their role in functional albumen deposition within the magnum, a phenomenon not observed in the ceased-laying ducks. Moreover, the proportion of THY1+ cells within the ECM-producing fibroblasts was found to be significantly higher in the egg-laying ducks (59%) compared to the ceased-laying ducks (24%). Similarly, TIMP4+ fibroblasts constituted a greater proportion of the ECM-producing fibroblasts in the egg-laying ducks (83%) compared to the ceased-laying ducks (58%). These findings suggest a potential correlation between the expression of THY1 and TIMP4 in ECM-producing fibroblasts and oviduct activity during functional reproduction. Our study provides valuable single-cell insights that warrant further investigation into the biological implications of fibroblast subsets in the degeneration of the reproductive tract. Moreover, these insights hold promise for enhancing the production efficiency of laying ducks.